Arabidopsis noncoding RNA modulates seedling greening during deetiolation

Arabidopsis noncoding RNA modulates seedling greening during deetiolation
复制标题

拟南芥非编码RNA在脱黄过程中调节幼苗绿化

DOI:
10.1007/s11427-017-9187-9
复制
发表时间:
2018-02
期刊:
Sci China Life Sci
影响因子:
--
通讯作者:
Zhu Danmeng
Zhu Danmeng
中科院分区:
其他
文献类型:
--
作者:
Wang Yuqiu;Li Jian;Deng Xing Wang;Zhu Danmeng

文献摘要

参考文献

相似文献

幼苗绿化对于从土壤中生长出来的植物的生存至关重要。叶绿素前体的丰度,包括原叶绿素(Pchlide),在黑暗到光明的过渡期间被精确控制,因为Pchlide的过度积累会导致细胞光氧化损伤。以前的研究已经确定并表征了控制这一重要过程的多个调节器。Hidden Treasure 1(隐藏的宝藏1)是在光形态建成中发现的第一个非编码RNA(ncRNA)。在连续红光下,HID1已显示通过抑制PIF3(光敏色素相互作用因子3)的转录来抑制下胚轴伸长。在这里,我们报告说,HID1作为子叶绿化的负调节。当暴露于白色光时,HID1的敲除导致黄化幼苗相对于野生型的绿化率增加。在遗传学上,HID1在暗到亮的转变期间作用于PIF3的下游。在黑暗中,HID1的表达不受PIF3的调节。分子上,Pchlide含量在黑暗生长的hid1突变体中比WT减少。与此同时,转录水平的protochlorophyllide氧化还原酶已知催化Pchlide叶绿素转化显着增加hid1幼苗。因此,我们的研究揭示了HID1在拟南芥暗到光的转换中的额外作用。此外,这些结果表明HID1可以调节不同光介导的发育过程中的不同靶点,因此对于控制这些机制至关重要。
Seedling greening is essential for the survival of plants emerging from the soil. The abundance of chlorophyll precursors, including protochlorophyllide (Pchlide), is precisely controlled during the dark-to-light transition, as over-accumulation of Pchlide can lead to cellular photooxidative damage. Previous studies have identified and characterized multiple regulators controlling this important process. HID1 (hidden treasure 1) is the first noncoding RNA (ncRNA) found in photomorphogenesis. Under continuous red light, HID1 has been shown to inhibit hypocotyl elongation by repressing the transcription of PIF3 (phytochrome interacting factor 3). Here, we report that HID1 acts as a negative regulator of cotyledon greening. Knockdown of HID1 resulted in an increased greening rate of etiolated seedlings relative to wild type when exposed to white light. Genetically, HID1 acts downstream of PIF3 during the dark-to-light transition. The expression of HID1 is not regulated by PIF3 in the dark. Molecularly, the Pchlide content was reduced in dark-grown hid1 mutants than WT. Meanwhile, transcript levels of the protochlorophyllide oxidoreductases known to catalyze Pchlide to chlorophyllide conversion were significantly increased in hid1 seedlings. Thus, our study reveals an additional role of HID1 in the dark-to-light transition in Arabidopsis. Moreover, these results suggest HID1 could regulate distinct targets in different light-mediated developmental processes, and thus is essential to the control of these mechanisms.
拟南芥非编码RNA介导红光对光形态发生的控制
DOI: 10.1073/pnas.1409457111
发表时间: 2014-06
期刊: PNAS
影响因子: --
作者:
He, Guangming;Terzaghi, William;Zhu, Danmeng;Deng, Xing Wang
通讯作者: Deng, Xing Wang
DOI: 10.1073/pnas.0907670106
发表时间: 2009-12-15
影响因子: 11.1
作者:
Zhong, Shangwei;Zhao, Mantong;Guo, Hongwei
通讯作者: Guo, Hongwei
拟南芥中中等大小非编码RNA的基因组特征和调控作用
DOI: 10.1093/mp/sst177
发表时间: 2014-03-01
期刊: MOLECULAR PLANT
影响因子: 27.5
作者:
Wang, Yuqiu;Wang, Xuncheng;Deng, Xing Wang
通讯作者: Deng, Xing Wang
DOI: 10.1073/pnas.0812219106
发表时间: 2009-05-05
影响因子: 11.1
作者:
Shin, Jieun;Kim, Keunhwa;Choi, Giltsu
通讯作者: Choi, Giltsu
RNA在意外的地方:长的非编码RNA在不同的细胞环境中起作用。
DOI: 10.1038/nrm3679
发表时间: 2013-11
期刊: Nature reviews. Molecular cell biology
影响因子: --
作者:
Geisler S;Coller J
通讯作者: Coller J