A rice calcium-dependent protein kinase is expressed in cortical root cells during the presymbiotic phase of the arbuscular mycorrhizal symbiosis.
A rice calcium-dependent protein kinase is expressed in cortical root cells during the presymbiotic phase of the arbuscular mycorrhizal symbiosis.
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在羊膜菌根菌根共生的前生物相期间，在皮质根细胞中表达水稻钙依赖性蛋白激酶。
DOI：
10.1186/1471-2229-11-90
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发表时间：
2011-05-19
影响因子：
5.3
通讯作者：
San Segundo B
中科院分区：
文献类型：
--
作者：
Campos-Soriano L;Gómez-Ariza J;Bonfante P;San Segundo B
丛枝菌根(AM)共生是土壤真菌与大多数植物根系之间的一种互惠共生关系。这种结合为丛枝菌根真菌提供了糖分，而真菌则改善了宿主植物对水分和矿物质营养的吸收。因此，丛枝菌根(AM)共生的建立需要微调宿主基因的表达，以识别和适应真菌共生体。在植物中，钙在发育过程和对环境刺激的反应中起着第二信使的关键作用。即使已知在AM共生期间宿主细胞中会发生钙瞬变，但对钙信号的解码和下游的分子事件仍然知之甚少。在AM共生的前共生阶段，监测了代表水稻CPK四个不同系统发育群的17个钙依赖蛋白激酶(CPK)基因的表达。其中，OsCPK18和OsCPK4被发现被AM真菌Glomus inaradices接种后在转录上被激活。真菌产生的可扩散分子也上调了OsCPK18和OsCPK4基因的表达。激光显微切割显示，OsCPK18基因在接种水稻根的皮层细胞中表达，而在表皮细胞中不表达，这表明该基因在根皮质中具有优先作用。此外，通过绿色荧光蛋白标记的OsCPK18在洋葱表皮细胞中的瞬时表达分析，观察到OsCPK18的质膜定位。我们还表明，OsCPK18 N末端的肉豆蔻酰化位点是质膜靶向所必需的。在AM接种后，OsCPK18的表达迅速激活，它的表达也被真菌分泌的信号诱导，再加上观察到的OsCPK18的质膜定位，表明OsCPK18在感知AM真菌方面发挥了作用。OsCPK18基因可能被认为是共生过程中前共生阶段的标志。这些发现有助于更好地理解AM共生过程中的信号机制，并将极大地促进AM共生的分子解剖。
The arbuscular mycorrhizal (AM) symbiosis consists of a mutualistic relationship between soil fungi and roots of most plant species. This association provides the arbuscular mycorrhizal fungus with sugars while the fungus improves the uptake of water and mineral nutrients in the host plant. Then, the establishment of the arbuscular mycorrhizal (AM) symbiosis requires the fine tuning of host gene expression for recognition and accommodation of the fungal symbiont. In plants, calcium plays a key role as second messenger during developmental processes and responses to environmental stimuli. Even though calcium transients are known to occur in host cells during the AM symbiosis, the decoding of the calcium signal and the molecular events downstream are only poorly understood. The expression of seventeen Calcium-dependent Protein Kinase (CPK) genes representative of the four distinct phylogenetic groups of rice CPKs was monitored during the presymbiotic phase of the AM symbiosis. Among them, OsCPK18 and OsCPK4, were found to be transcriptionally activated in response to inoculation with the AM fungus Glomus intraradices. OsCPK18 and OsCPK4 gene expression was also up-regulated by fungal-produced diffusible molecules. Laser microdissection revealed expression of OsCPK18 in cortical cells, and not in epidermal cells of G. intraradices-inoculated rice roots, suggesting a preferential role of this gene in the root cortex. Moreover, a plasma membrane localization of OsCPK18 was observed by transient expression assays of green fluorescent protein-tagged OsCPK18 in onion epidermal cells. We also show that the myristoylation site of the OsCPK18 N-terminus is required for plasma membrane targeting. The rapid activation of OsCPK18 expression in response to AM inoculation, its expression being also induced by fungal-secreted signals, together with the observed plasma membrane localization of OsCPK18, points to a role for OsCPK18 in perception of the AM fungus. The OsCPK18 gene might be considered as a marker for the presymbiotic phase of the symbiotic process. These findings provide a better understanding of the signaling mechanisms operating during the AM symbiosis and will greatly facilitate their molecular dissection.
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影响因子：
64.8
作者：
HARRISON, MJ;VANBUUREN, ML
通讯作者：
VANBUUREN, ML
影响因子：
11.6
作者：
Genre, A;Chabaud, M;Barker, DG
通讯作者：
Barker, DG
影响因子：
7.4
作者：
Karas, Bogumil;Amyot, Lisa;Szczyglowski, Krzysztof
通讯作者：
Szczyglowski, Krzysztof
DOI：
10.1073/pnas.0508883103
发表时间：
2006-01-10
影响因子：
11.1
作者：
Kanamori, N;Madsen, LH;Stougaard, J
通讯作者：
Stougaard, J
影响因子：
7.4
作者：
Chen, Caiyan;Gao, Muqiang;Zhu, Hongyan
通讯作者：
Zhu, Hongyan