Specificity and function of archaeal DNA replication initiator proteins.
Specificity and function of archaeal DNA replication initiator proteins.
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DOI：
10.1016/j.celrep.2013.01.002
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发表时间：
2013-02-21
期刊：
影响因子：
8.8
通讯作者：
Bell SD
中科院分区：
文献类型：
--
作者：
Samson RY;Xu Y;Gadelha C;Stone TA;Faqiri JN;Li D;Qin N;Pu F;Liang YX;She Q;Bell SD
具有多个DNA复制起点的染色体是真核生物和某些微生物的标志。所有真核细胞核复制起点都由起点识别复合物（ORC）定义，该复合物通过Cdc 6和Cdt 1募集复制解旋酶MCM（2-7）。我们发现，在单一染色体的古硫化叶菌islandicus的三个起源是由不同的起始因子。虽然两个起源依赖于真核生物Orc 1和Cdc 6的古细菌同源物，但第三个起源反而依赖于古细菌Cdt 1同源物。我们利用orc 1 -1基因的非必需性质来研究ATP结合和水解在体内和体外引发功能中的作用。我们发现，ATP结合形式的Orc 1 -1是熟练的复制和牵连的ATP水解下调的起源活动。最后，我们揭示了ATP和DNA结合Orc 1 -1重塑蛋白质的结构，而不是DNA模板。冰岛硫化叶菌染色体有三个起源，每个起源都有自己的起始点。两个起源依赖于Orc，一个需要Cdt 1同源物。Orc 1的ATP结合形式擅长MCM加载，ATP结合重塑蛋白质结构，而不是DNA模板。硫化叶菌属的每个染色体使用三个复制起点。她、Bell和同事表明，岛鼠的三个起源具有不同的启动子蛋白，使该染色体成为复制子的马赛克。Orc 1/Cdc 6基因的非必需性质允许结合体外和体内分析其功能。这些发现表明，ATP结合，而不是水解，是Orc 1功能所必需的，ATP通过重塑起始蛋白而不是原始DNA来发挥其作用。
Chromosomes with multiple DNA replication origins are a hallmark of Eukaryotes and some Archaea. All eukaryal nuclear replication origins are defined by the origin recognition complex (ORC) that recruits the replicative helicase MCM(2-7) via Cdc6 and Cdt1. We find that the three origins in the single chromosome of the archaeon Sulfolobus islandicus are specified by distinct initiation factors. While two origins are dependent on archaeal homologs of eukaryal Orc1 and Cdc6, the third origin is instead reliant on an archaeal Cdt1 homolog. We exploit the nonessential nature of the orc1-1 gene to investigate the role of ATP binding and hydrolysis in initiator function in vivo and in vitro. We find that the ATP-bound form of Orc1-1 is proficient for replication and implicates hydrolysis of ATP in downregulation of origin activity. Finally, we reveal that ATP and DNA binding by Orc1-1 remodels the protein’s structure rather than that of the DNA template. ► The S. islandicus chromosome has three origins, each with its own initiator ► Two origins are Orc dependent, and one requires a Cdt1 homolog ► The ATP-bound form of Orc1 is proficient at MCM loading ► ATP binding remodels the protein structure, not that of the DNA template Archaea of the genus Sulfolobus use three replication origins per chromosome. She, Bell, and colleagues show that the three origins in S. islandicus have distinct initiator proteins, making this chromosome a mosaic of replicons. The nonessential nature of the Orc1/Cdc6 genes permits combined in vitro and in vivo analyses of their function. These findings reveal that ATP binding, not hydrolysis, is required for Orc1 function and that ATP exerts its effect by remodeling the initiator protein, not the origin DNA.
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影响因子：
7.8
作者：
Kaguni, Jon M.
通讯作者：
Kaguni, Jon M.
通过细菌启动器拉伸的DNA促进了复制起源。
影响因子：
64.8
作者：
Duderstadt, Karl E.;Chuang, Kevin;Berger, James M.
通讯作者：
Berger, James M.
DOI：
10.1098/rstb.2011.0073
发表时间：
2011-12-27
期刊：
Philosophical transactions of the Royal Society of London. Series B, Biological sciences
影响因子：
--
作者：
Diffley JF
通讯作者：
Diffley JF
影响因子：
11.4
作者：
Robinson, Nicholas P.;Blood, Katherine A.;Bell, Stephen D.
通讯作者：
Bell, Stephen D.
影响因子：
3.2
作者：
Frols, Sabrina;Gordon, Paul M. K.;Schleper, Christa
通讯作者：
Schleper, Christa