ShK-Domain-Containing Protein from a Parasitic Nematode Modulates Drosophila melanogaster Immunity.
ShK-Domain-Containing Protein from a Parasitic Nematode Modulates Drosophila melanogaster Immunity.
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DOI：
10.3390/pathogens11101094
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发表时间：
2022-09-24
期刊：
影响因子：
--
通讯作者：
Dillman AR
中科院分区：
文献类型：
--
作者：
Lima AK;Dhillon H;Dillman AR
理解宿主-寄生虫相互作用的一个关键组成部分是宿主和寄生虫之间的分子串扰。寄生线虫分泌的排泄物在寄生过程中起着重要作用。它们可以直接损害宿主组织并调节宿主防御。Steinernema carpocapsae是一种研究充分的昆虫寄生虫，在感染过程中释放约500种毒液蛋白。虽然这些蛋白质的身份是已知的，但很少有人详细研究。存在于由这些线虫释放的ESP中的一个蛋白质家族是ShK家族。我们研究了S. Carpocapsae ESP，Sc-ShK-1，以研究其在果蝇模型中的作用。我们发现Sc-ShK-1在高应激条件下是有毒的，并对果蝇的健康产生负面影响。我们已经表明，Sc-ShK-1有助于宿主在细菌合并感染中的免疫调节，导致死亡率和微生物生长增加。这项研究提供了一个深入了解ShK结构域的蛋白质从线虫，并建议这些蛋白质可能在宿主-寄生虫相互作用中发挥重要作用。
A key component to understanding host–parasite interactions is the molecular crosstalk between host and parasite. Excreted/secreted products (ESPs) released by parasitic nematodes play an important role in parasitism. They can directly damage host tissue and modulate host defense. Steinernema carpocapsae, a well-studied parasite of insects releases approximately 500 venom proteins as part of the infection process. Though the identity of these proteins is known, few have been studied in detail. One protein family present in the ESPs released by these nematodes is the ShK family. We studied the most abundant ShK-domain-containing protein in S. carpocapsae ESPs, Sc-ShK-1, to investigate its effects in a fruit fly model. We found that Sc-ShK-1 is toxic under high stress conditions and negatively affects the health of fruit flies. We have shown that Sc-ShK-1 contributes to host immunomodulation in bacterial co-infections resulting in increased mortality and microbial growth. This study provides an insight on ShK-domain-containing proteins from nematodes and suggests these proteins may play an important role in host–parasite interactions.
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影响因子：
4.6
作者：
Jones, Kirah;Tafesh-Edwards, Ghada;Kenney, Eric;Toubarro, Duarte;Simoes, Nelson;Eleftherianos, Ioannis
通讯作者：
Eleftherianos, Ioannis
影响因子：
3.5
作者：
Balasubramanian, Natesan;Nascimento, Gisela;Simoes, Nelson
通讯作者：
Simoes, Nelson
影响因子：
3.7
作者：
Linderman JA;Chambers MC;Gupta AS;Schneider DS
通讯作者：
Schneider DS
影响因子：
4.2
作者：
Gerdol, Marco;Cervelli, Manuela;Modica, Maria Vittoria
通讯作者：
Modica, Maria Vittoria
影响因子：
4.8
作者：
Chhabra, Sandeep;Chang, Shih Chieh;Chandy, K. George
通讯作者：
Chandy, K. George