High-throughput measurements of hydrogel tissue construct mechanics.
High-throughput measurements of hydrogel tissue construct mechanics.
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DOI：
10.1089/ten.tec.2008.0347
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发表时间：
2009-06
期刊：
影响因子：
--
通讯作者：
Wakatsuki T
中科院分区：
文献类型：
--
作者：
Marquez JP;Legant W;Lam V;Cayemberg A;Elson E;Wakatsuki T
Engineered tissues represent a natural environment for studying cell physiology, mechanics, and function. Cellular interactions with the extracellular matrix proteins are important determinants of cell physiology and tissue mechanics. Dysregulation of these parameters can result in diseases such as cardiac fibrosis and atherosclerosis. In this report we present a novel system to produce hydrogel tissue constructs (HTCs) and to characterize their mechanical properties. HTCs are grown in custom chambers and a robotic system is used to indent them and measure the resulting forces. Force measurements are then used to estimate HTC pretension (cellular contractility). Pretension was reduced in a dose-dependent manner by cytochalasin D (CD) treatment; the highest concentration (2 μM) resulted in ~10-fold decrease. On the other hand, treatment with fetal bovine serum (20%) resulted in approximately threefold increase in pretension. Excellent repeatability and precision were observed in measurements from replicate HTCs. The coefficient of statistical variance of quantified pretension ranged from 7% to 15% (n = 4). Due to the small size (4×4×0.8 mm) of the HTCs, this system of profiling HTC mechanics can readily be used in high-throughput applications. In particular, it can be used for screening chemical libraries in search of drugs that can alter tissue mechanics.
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影响因子：
2.2
作者：
TRANQUILLO, RT;DURRANI, MA;MOON, AG
通讯作者：
MOON, AG
DOI：
10.1083/jcb.117.1.73
发表时间：
1992-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kolodney MS;Wysolmerski RB
通讯作者：
Wysolmerski RB
影响因子：
56.9
作者：
LANGER, R;VACANTI, JP
通讯作者：
VACANTI, JP
DOI：
10.1016/0304-4165(94)90040-x
发表时间：
1994-11-11
影响因子：
3
作者：
EASTWOOD, M;MCGROUTHER, DA;BROWN, RA
通讯作者：
BROWN, RA
影响因子：
--
作者：
TOMASEK, JJ;HAAKSMA, CJ;VAUGHAN, MB
通讯作者：
VAUGHAN, MB