A simple medium enables bovine embryos to be held for seven days at 4°C.
A simple medium enables bovine embryos to be held for seven days at 4°C.
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DOI：
10.1038/srep01173
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发表时间：
2013
影响因子：
4.6
通讯作者：
Tsuda, Sakae
中科院分区：
文献类型：
--
作者：
Ideta, Atsushi;Aoyagi, Yoshito;Tsuchiya, Kanami;Kamijima, Tatsuro;Nishimiya, Yoshiyuki;Tsuda, Sakae
利用液氮（液氮）冷冻保存配子和胚胎的方法在哺乳动物人工繁殖中是普遍的。然而，在过去的二十年里，冷冻胚胎的怀孕率并没有提高，因为冷冻-解冻会造成严重的损害。航空公司对液氮集装箱运输的严格管制也限制了育种者之间的交流。在这篇文章中，我们介绍了一种能够使牛胚胎在4°C下保持长达7天的培养基。胚胎在此培养液中培养7天后移植到预处理受体，妊娠率为75%（24/32）。其成分为199培养基、胎牛血清和HEPES缓冲液。这项技术将使胚胎的无液氮储存和空运能够在7天内完成移植给受体。
Cryopreservation methods using liquid nitrogen (LN2) for gametes and embryos are prevalent in mammalian artificial reproduction. However, the pregnancy rate from frozen embryos has not improved over the past two decades because freeze–thawing causes significant damage. The strict regulation of transportation of LN2 containers by airlines also limits exchange between breeders. In this article, we introduce a medium that enabled bovine embryos to be held for up to 7 days at 4°C. A pregnancy rate of 75% (24/32) was obtained for embryos held for 7 days in this medium and transferred to primed recipients. Its constituents were medium 199, foetal bovine serum, and HEPES for buffering. This technique will enable LN2-free storage and air transportation of embryos provided transplantation to recipients can be completed within 7 days.
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