Cystatin SN upregulation in patients with seasonal allergic rhinitis.
Cystatin SN upregulation in patients with seasonal allergic rhinitis.
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DOI：
10.1371/journal.pone.0067057
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Fujieda S
中科院分区：
文献类型：
--
作者：
Imoto Y;Tokunaga T;Matsumoto Y;Hamada Y;Ono M;Yamada T;Ito Y;Arinami T;Okano M;Noguchi E;Fujieda S
季节性变应性鼻炎（SAR）是一种由IgE介导的影响鼻粘膜的I型变态反应。然而，其发展背后的分子事件仍不清楚。我们试图在自然暴露于JC花粉的鼻上皮细胞中鉴定SAR相关的基因表达改变。我们在2009年和2010年招募了研究参与者，并在2月至4月期间收集了鼻上皮细胞，这是自然花粉散布的时期。2009年入组了15例SAR-JC患者和13例对照受试者，2010年入组了17例SAR-JC患者、13例致敏无症状受试者（致敏）和15例对照受试者。2009年，从鼻上皮细胞中提取总RNA，并使用Illumina HumanRef-8表达BeadChip平台对8名SAR-JC患者和6名对照受试者进行了微阵列分析。从SAR患者分离的外周血嗜碱性粒细胞中检测过敏原刺激的组胺释放。我们确定了32个基因在过敏原暴露过程中表达显著改变。其中之一，CST 1编码半胱氨酸蛋白酶抑制剂，半胱氨酸蛋白酶抑制剂SN。与对照组相比，2009年和2010年SAR-JC组鼻上皮细胞中的CST 1表达显著上调。免疫组织化学染色证实，在SAR患者的鼻上皮细胞的表达增加。在体外，向嗜碱性粒细胞中加入外源性CST 1抑制JC变应原刺激的组胺释放。我们认为，CST 1可能有助于蛋白酶过敏原的失活，并帮助重建鼻膜的稳态。
Seasonal allergic rhinitis (SAR) to the Japanese cedar, Cryptomeria japonica (JC) pollen is an IgE-mediated type I allergy affecting nasal mucosa. However, the molecular events underlying its development remain unclear. We sought to identify SAR-associated altered gene expression in nasal epithelial cells during natural exposure to JC pollen. We recruited study participants in 2009 and 2010 and collected nasal epithelial cells between February and April, which is the period of natural pollen dispersion. Fifteen patients with SAR-JC and 13 control subjects were enrolled in 2009, and 17 SAR-JC patients, 13 sensitized asymptomatic subjects (Sensitized), and 15 control subjects were enrolled in 2010. Total RNA was extracted from nasal epithelial cells and 8 SAR-JC patients and 6 control subjects in 2009 were subjected to microarray analysis with the Illumina HumanRef-8 Expression BeadChip platform. Allergen-stimulated histamine release was examined in the peripheral blood basophils isolated from patients with SAR. We identified 32 genes with significantly altered expression during allergen exposure. One of these, CST1 encodes the cysteine protease inhibitor, cystatin SN. CST1 expression in nasal epithelial cells was significantly upregulated in both the 2009 and 2010 SAR-JC groups compared with the control groups. Immunohistochemical staining confirmed the increased expression of CST1 in the nasal epithelial cells of SAR patients. Addition of exogenous CST1 to basophils inhibited JC allergen-stimulated histamine release in vitro. We propose that CST1 may contribute to inactivation of protease allergens and help re-establish homeostasis of the nasal membranes.
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影响因子：
24.3
作者：
Giovannini-Chami, Lisa;Marcet, Brice;Barbry, Pascal
通讯作者：
Barbry, Pascal
DOI：
10.1006/bbrc.1996.0544
发表时间：
1996-04-05
影响因子：
3.1
作者：
Kalsheker, NA;Deam, S;Lomas, DA
通讯作者：
Lomas, DA
影响因子：
2.8
作者：
Sakashita, Masafumi;Hirota, Tomomitsu;Fujieda, Shigeharu
通讯作者：
Fujieda, Shigeharu
DOI：
10.1165/rcmb.2003-0060oc
发表时间：
2003-09-01
影响因子：
6.4
作者：
Brown, A;Farmer, K;Sallenave, JM
通讯作者：
Sallenave, JM
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y