The transverse aortic constriction heart failure animal model: a systematic review and meta-analysis.
The transverse aortic constriction heart failure animal model: a systematic review and meta-analysis.
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横向主动脉收缩心力衰竭动物模型：系统评价和荟萃分析。
DOI：
10.1007/s10741-020-09960-w
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发表时间：
2021-11
影响因子：
4.6
通讯作者：
de Jager SCA
中科院分区：
文献类型：
--
作者：
Bosch L;de Haan JJ;Bastemeijer M;van der Burg J;van der Worp E;Wesseling M;Viola M;Odille C;El Azzouzi H;Pasterkamp G;Sluijter JPG;Wever KE;de Jager SCA
横主动脉缩窄（TAC）模型常用于研究压力超负荷时的不良心脏重构。我们开始定义在该模型中定义心脏重构程度的最重要特征。对使用TAC小鼠/大鼠模型并报告超声心动图结局参数的研究进行了系统综述和荟萃分析。我们纳入了所有动物研究，其中评估了横主动脉周围的缩窄和至少一个预定义的超声心动图或MRI结果参数。总共502篇文章和> 3000只接受TAC的野生型未治疗动物被纳入本研究，并参考对照组。主动脉缩窄的持续时间与不良重构的程度相关。然而，由于优先使用雄性C57 B1/6小鼠（66%的研究），小鼠数据存在强烈偏倚。此外，主要使用氯胺酮/甲苯噻嗪麻醉剂、27 G针收缩和丝线缝合。尽管如此，尽管实验设计的同质性，该模型包含了相当程度的异质性的功能结果的措施。当观察研究质量时，只有12%的人报告了随机化，23%的人提到了任何形式的盲法，25%的人充分讨论了结果，并且令人惊讶的低百分比（2%）显示了样本量计算。荟萃分析未检测到解释报告结局指标异质性的特定研究特征，但这可能与使用特定小鼠品系、性别和年龄的强烈偏倚或研究质量特征报告不佳有关。本文的在线版本（10.1007/s10741-020-09960-w）包含补充材料，可供授权用户使用。
The transverse aortic constriction (TAC) model is frequently used to study adverse cardiac remodeling upon pressure overload. We set out to define the most important characteristics that define the degree of cardiac remodeling in this model. A systematic review and meta-analyses were performed on studies using the TAC mouse/rat model and reporting echocardiographic outcome parameters. We included all animal studies in which a constriction around the transverse aorta and at least one of the predefined echocardiography or MRI outcome parameters were assessed. A total of 502 articles and > 3000 wild-type, untreated animals undergoing TAC were included in this study and referenced to a control group. The duration of aortic constriction correlated to the degree of adverse remodeling. However, the mouse data is strongly biased by the preferential use of male C57Bl/6 mice (66% of studies). Furthermore, mostly ketamine/xylazine anesthetics, 27G needle constriction, and silk sutures are used. Nonetheless, despite the homogeneity in experimental design, the model contained a substantial degree of heterogeneity in the functional outcome measures. When looking at study quality, only 12% reported randomization, 23% mentioned any sort of blinding, 25% adequately addressed the outcomes, and an amazingly low percentage (2%) showed sample size calculation. Meta-analyses did not detect specific study characteristics that explained the heterogeneity in the reported outcome measures, however this might be related to the strong bias towards the use of specific mouse lines, sex as well as age or to poor reporting of characteristics of study quality. The online version of this article (10.1007/s10741-020-09960-w) contains supplementary material, which is available to authorized users.
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影响因子：
2.4
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Hooijmans CR;Tillema A;Leenaars M;Ritskes-Hoitinga M
通讯作者：
Ritskes-Hoitinga M
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Bui, Anh L.;Horwich, Tamara B.;Fonarow, Gregg C.
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KARP, RB
影响因子：
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