Coordinate regulation of gluconeogenesis by the glucocorticoids and glucagon: Evidence for acute and chronic regulation by glucagon
Coordinate regulation of gluconeogenesis by the glucocorticoids and glucagon: Evidence for acute and chronic regulation by glucagon
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糖皮质激素和胰高血糖素对糖异生的协调调节：胰高血糖素急性和慢性调节的证据
DOI：
10.1002/jcp.1041090110
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发表时间：
1981
影响因子：
5.6
通讯作者：
D. Stumpo
中科院分区：
文献类型：
--
作者：
R. Kletzien;Charles A. Weber;D. Stumpo
本文研究了糖皮质激素和胰高血糖素对原代培养的成年大鼠肝实质细胞肝纤维化的协同调节作用。结果表明，胰高血糖素刺激3碳前体产生葡萄糖至少由两种糖皮质激素不同影响的组分组成。胰高血糖素处理肝细胞导致葡萄糖产生立即增加，其不被放线菌酮阻断，并且在没有任何可检测的磷酸烯醇丙酮酸羧激酶活性增加的情况下发生。该组分似乎受到翻译后机制的调节，并涉及将碳流从糖酵解重定向到糖异生。第二个组成部分的特征是需要长期胰高血糖素治疗。这种葡萄糖产生的增加可以被放线菌酮阻断，并且与磷酸烯醇丙酮酸羧激酶活性的增加相关。长期胰高血糖素孵育加速的反应位于磷酸丙糖水平之前，因为与短期孵育相比，胰高血糖素长期孵育不能增加二羟基丙酮的葡萄糖产量。这表明磷酸烯醇丙酮酸羧激酶而不是氨基酸转运是长期孵育中的关键起搏反应，因为无论丙氨酸或乳酸盐用作3碳前体，糖皮质激素和胰高血糖素刺激葡萄糖产生的反应方向和幅度都是相同的。糖皮质激素对胰高血糖素刺激的第一组分的葡萄糖产生表现出累加效应，而它们放大了第二组分。
The coordinate regulation of gluconeogenesis by the glucocorticoids and glucagon in primary cultures of adult rat liver parenchymal cells has been studied. The results suggest that glucagon stimulation of glucose production from 3‐carbon precursors is composed of at least two components which the glucocorticoids differentially affect. Glucagon treatment of hepatocytes results in an immediate increase in glucose production which is not blocked by cycloheximide and occurs in the absence of any detectable increase of phosphoenolpyruvate carboxykinase activity. This component appears to be regulated by a post‐translational mechanism and involves redirection of carbon flow from glycolysis to gluconeogenesis. The second component is characterized by the need for long‐term glucagon treatment. This increase in glucose production can be blocked by cycloheximide and is correlated with an increase in phosphoenolpyruvate carbooxykinase activity. The reaction that is accelerated by long‐term glucagon incubation is located prior to the triose‐phosphate level since long‐term incubation with glucagon fails to increase glucose production from dihydroxyacetone any more than does short‐term incubation. It is suggested that phosphoenolpyruvate carboxykinase rather than amino acid transport is the key pacemaker reaction in the long‐term incubation since the direction and magnitude of the response for glucocorticoid and glucagon stimulation of glucose production is the same whether alanine or lactate is used as the 3‐carbon precursor. The glucocorticoids exhibit an additive effect on glucagon‐stimulated glucose production for the first component whereas they amplify the second component.
由磷酸和果糖 6-磷酸合成磷酸果糖激酶刺激剂，最有可能是果糖 2,6-二磷酸。
DOI：
10.1016/0006-291x(80)91347-9
发表时间：
1980
影响因子：
3.1
作者：
VanSchaftingen,E;Hers,HG
通讯作者：
Hers,HG
肝细胞中磷酸果糖激酶激活因子浓度对葡萄糖和胰高血糖素的反应发生变化。
DOI：
10.1016/s0006-291x(80)80040-4
发表时间：
1980
影响因子：
3.1
作者：
Richards,CS;Uyeda,K
通讯作者：
Uyeda,K
大鼠肝脏中磷酸烯醇丙酮酸羧激酶 (GTP) mRNA 周转的调节。
DOI：
--
发表时间：
1980
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Nelson,K;Cimbala,MA;Hanson,RW
通讯作者：
Hanson,RW