Equine tendonitis therapy using mesenchymal stem cells and platelet concentrates: a randomized controlled trial.
Equine tendonitis therapy using mesenchymal stem cells and platelet concentrates: a randomized controlled trial.
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DOI：
10.1186/scrt236
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发表时间：
2013-07-22
影响因子：
7.5
通讯作者：
Garcia Alves AL
中科院分区：
文献类型：
--
作者：
Carvalho Ade M;Badial PR;Álvarez LE;Yamada AL;Borges AS;Deffune E;Hussni CA;Garcia Alves AL
肌腱损伤是运动马跛行和表现下降的主要原因。已经描述了多种肌腱炎的治疗方法；然而，这些疗法都无法实现组织的完全再生，而且即使经过长时间的休息和物理治疗恢复，损伤复发率也很高。用胶原酶凝胶在八只混合品种马的掌骨区域中心部分的浅表屈肌腱中诱导病变。两周后，治疗组和对照组动物的病灶分别通过病灶内注射悬浮于浓缩血小板（PC）中的脂肪组织间充质干细胞（adMSCs）和磷酸盐缓冲盐水（PBS）来治疗。每两周进行一次系列超声分析。治疗 16 周后，进行活检以进行组织病理学、免疫组织化学和基因表达（I 型胶原蛋白 (COL1A1)、III 型胶原蛋白 (COL3A1)、腱蛋白-C (TNC)、腱调节蛋白 (TNMD) 和硬化剂 (SCX)）分析。观察到各组之间超声和组织病理学分析的差异。据报告，用悬浮在 PC 中的 adMSC 治疗组的结果有所改善。不同处理后观察到的基因表达水平没有差异。从治疗组的组织病理学评估中观察到的主要结果如下：预防病变进展、胶原纤维组织增多以及炎症浸润减少。在超声图像中观察到病变区域及其百分比没有进展，并且通过能量多普勒测量血流量增加。正如超声检查中所观察到的那样，使用adMSCs与PC相结合来治疗实验诱发的肌腱炎可以阻止肌腱病变的进展，并且可以如组织病理学评估中所观察到的那样，导致更大的组织和减少的炎症。这些数据证明了该疗法治疗马肌腱炎的治疗潜力。
Tendon injury is a major cause of lameness and decreased performance in athletic equines. Various therapies for tendonitis have been described; however, none of these therapies results in complete tissue regeneration, and the injury recurrence rate is high even after long recovery periods involving rest and physiotherapy. A lesion was induced with collagenase gel in the superficial digital flexor tendon in the center portion of the metacarpal region of eight equines of mixed breed. After two weeks, the lesions of the animals in the treated and control groups were treated through the intralesional administration of mesenchymal stem cells derived from adipose tissue (adMSCs) suspended in platelet concentrate (PC) and with phosphate buffered saline (PBS), respectively. Serial ultrasound analyses were performed every two weeks. After 16 weeks of therapy, a biopsy was performed for histopathological, immunohistochemical and gene expression (type I collagen (COL1A1), type III collagen (COL3A1), tenascin-C (TNC), tenomodulin (TNMD), and scleraxis (SCX)) analyses. Differences in the ultrasound and histopathological analyses were observed between the groups. Improved results were reported in the group treated with adMSCs suspended in PC. There was no difference in the gene expression levels observed after the different treatments. The main results observed from the histopathological evaluation of the treated group were as follows: a prevention of the progression of the lesion, a greater organization of collagen fibers, and a decreased inflammatory infiltrate. A lack of progression of the lesion area and its percentage was observed in the ultrasound image, and increased blood flow was measured by Power Doppler. The use of adMSCs combined with PC for the therapy of experimentally induced tendonitis prevented the progression of the tendon lesion, as observed in the ultrasound examination, and resulted in a greater organization and decreased inflammation, as observed in the histopathological evaluation. These data demonstrate the therapeutic potential of this therapy for the treatment of equine tendonitis.
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影响因子：
2.2
作者：
Arguelles, D.;Carmona, J. U.;Prades, M.
通讯作者：
Prades, M.
影响因子：
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通讯作者：
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作者：
Carvalho, Armando de Mattos;Garcia Alves, Ana Liz;Deffune, Elenice
通讯作者：
Deffune, Elenice