OsHUS1 facilitates accurate meiotic recombination in rice.
OsHUS1 facilitates accurate meiotic recombination in rice.
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OsHUS1 促进水稻减数分裂的精确重组。
DOI：
10.1371/journal.pgen.1004405
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发表时间：
2014-06
期刊：
影响因子：
4.5
通讯作者：
Cheng Z
中科院分区：
文献类型：
--
作者：
Che L;Wang K;Tang D;Liu Q;Chen X;Li Y;Hu Q;Shen Y;Yu H;Gu M;Cheng Z
减数分裂重组通常发生在同源物上的等位基因序列之间。这个过程也可以发生在非等位基因同源序列之间。这种异位相互作用事件可导致染色体重排，通常是避免的。然而，对于这些异位相互作用事件是如何被感知和消除的，我们仍然知之甚少。在本研究中，我们利用水稻筛选，鉴定了HUS1的同源物，并探讨了其在减数分裂重组中的功能。在Oshus1突变体中，伴随着近乎正常的同源配对和突触，非同源染色体之间发生了剧烈的异常异位相互作用，导致多价形成和随后的染色体断裂。这些异位相互作用依赖于程序性减数分裂双链断裂，并以独立于osmer3介导的干扰敏感交叉途径的方式形成。虽然早期同源重组事件正常发生，但在缺乏OsHUS1的情况下，干扰敏感交叉的数量减少。总之，我们的研究结果表明，OsHUS1可能通过形成典型的RAD9-RAD1-HUS1（9-1-1）复合体参与了减数分裂过程中异位相互作用的调节。减数分裂是一种特殊类型的细胞分裂，产生配子进行有性生殖。在减数分裂过程中，重组不仅发生在同源基因上的等位基因序列之间，也发生在分散位点上的非等位基因同源序列之间。这种异位重组是染色体改变的主要原因，也是人类许多基因组疾病的原因。为了确保基因组的完整性，必须迅速解决这些异位重组。尽管异位重组抑制很重要，但这一过程的机制仍然很大程度上未知。本研究以水稻为模型系统，鉴定了水稻HUS1同源物（RAD9-RAD1-HUS1（9-1-1）复合体的成员），并阐明了其在减数分裂重组中的作用。在Oshus1中，非同源染色体之间发生强烈的异位相互作用，交叉数量减少。我们推测OsHUS1可能通过形成典型的RAD9-RAD1-HUS1（9-1-1）复合体参与了减数分裂过程中异位相互作用的调节。
Meiotic recombination normally takes place between allelic sequences on homologs. This process can also occur between non-allelic homologous sequences. Such ectopic interaction events can lead to chromosome rearrangements and are normally avoided. However, much remains unknown about how these ectopic interaction events are sensed and eliminated. In this study, using a screen in rice, we characterized a homolog of HUS1 and explored its function in meiotic recombination. In Oshus1 mutants, in conjunction with nearly normal homologous pairing and synapsis, vigorous, aberrant ectopic interactions occurred between nonhomologous chromosomes, leading to multivalent formation and subsequent chromosome fragmentation. These ectopic interactions relied on programed meiotic double strand breaks and were formed in a manner independent of the OsMER3-mediated interference-sensitive crossover pathway. Although early homologous recombination events occurred normally, the number of interference-sensitive crossovers was reduced in the absence of OsHUS1. Together, our results indicate that OsHUS1 might be involved in regulating ectopic interactions during meiosis, probably by forming the canonical RAD9-RAD1-HUS1 (9-1-1) complex. Meiosis is a special type of cell division that generates gametes for sexual reproduction. During meiosis, recombination not only occurs between allelic sequences on homologs, but also between non-allelic homologous sequences at dispersed loci. Such ectopic recombination is the main cause of chromosomal alterations and accounts for numerous genomic disorders in humans. To ensure genomic integrity, those ectopic recombinations must be quickly resolved. Despite the importance of ectopic recombination suppression, the mechanism underlying this process still remains largely unknown. Here, using rice as a model system, we identified the rice HUS1 homolog, a member of the RAD9-RAD1-HUS1 (9-1-1) complex, and elucidated its roles in meiotic recombination. In Oshus1, vigorous ectopic interactions occur between nonhomologous chromosomes, and the number of crossovers is reduced. We suspect that OsHUS1 participates in regulating ectopic interactions during meiosis, probably by forming the canonical RAD9-RAD1-HUS1 (9-1-1) complex.
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影响因子：
4.5
作者：
Da Ines O;Abe K;Goubely C;Gallego ME;White CI
通讯作者：
White CI
影响因子：
3.5
作者：
Hastings PJ
通讯作者：
Hastings PJ
影响因子：
10.5
作者：
ENOCH, T;CARR, AM;NURSE, P
通讯作者：
NURSE, P
DOI：
10.1073/pnas.97.17.9537
发表时间：
2000-08-15
影响因子：
11.1
作者：
Goldman, ASH;Lichten, M
通讯作者：
Lichten, M
影响因子：
7.2
作者：
Heitzeberg, F;Chen, IP;Puchta, H
通讯作者：
Puchta, H