Differing requirements for RAD51 and DMC1 in meiotic pairing of centromeres and chromosome arms in Arabidopsis thaliana.
Differing requirements for RAD51 and DMC1 in meiotic pairing of centromeres and chromosome arms in Arabidopsis thaliana.
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DOI：
10.1371/journal.pgen.1002636
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发表时间：
2012
期刊：
影响因子：
4.5
通讯作者：
White CI
中科院分区：
文献类型：
--
作者：
Da Ines O;Abe K;Goubely C;Gallego ME;White CI
在减数分裂过程中，同源染色体配对、重组和联会，从而确保精确的染色体分离和配子发生所需的倍性减半。允许染色体仅与其同源物配对的过程尚未完全了解，但同源染色体的成功配对与重组紧密相关。在拟南芥中，rad 51，xrcc 3，和rad 51 C突变体的减数分裂前期出现正常的偶线期/粗线期阶段，之后的基因组片段，导致不育。为了更好地理解重组和染色体配对之间的关系，我们分析了这些和dmc 1突变株系的减数分裂染色体配对。我们的数据显示，这些蛋白质的配对的着丝粒区域和染色体臂的不同要求。在rad 51，xrcc 3和rad 51 C突变体中没有观察到中臂或远端区域的同源配对。然而，同源着丝粒配对在这些突变体，我们表明，这取决于重组，主要是在DMC 1。这种着丝粒配对远远超出异染色质着丝粒区域，并且令人惊讶的是，不需要XRCC 3和RAD 51 C。除了澄清并将着丝粒在减数分裂突触中的作用摆在前面之外，该分析因此将DMC 1和RAD 51以及减数分裂RAD 51旁系同源物XRCC 3和RAD 51 C在减数分裂突触中的作用相对于不同的染色体结构域分开。减数分裂是一种特殊的细胞分裂，在真核生物有性生殖的配子产生中，其作用是使染色体互补或倍性减半。为了确保每个配子都有完整的遗传物质，同源染色体必须配对，然后在减数分裂期间以协调的方式分离，这在大多数研究的真核生物中是通过重组介导的。为了更好地理解重组和减数分裂同源配对之间的关系，我们分析了缺乏关键重组蛋白的植物突变体的减数分裂染色体配对。这项工作提供了新的见解拟南芥减数分裂前期发生的同源染色体配对机制：异染色质着丝粒和5S rDNA区域配对早，他们的配对有不同的重组蛋白的要求比染色体臂。这些数据提出了一些问题的特异性和作用的重组在不同的染色体和/或染色质区域的同源染色体在减数分裂的联会。
During meiosis homologous chromosomes pair, recombine, and synapse, thus ensuring accurate chromosome segregation and the halving of ploidy necessary for gametogenesis. The processes permitting a chromosome to pair only with its homologue are not fully understood, but successful pairing of homologous chromosomes is tightly linked to recombination. In Arabidopsis thaliana, meiotic prophase of rad51, xrcc3, and rad51C mutants appears normal up to the zygotene/pachytene stage, after which the genome fragments, leading to sterility. To better understand the relationship between recombination and chromosome pairing, we have analysed meiotic chromosome pairing in these and in dmc1 mutant lines. Our data show a differing requirement for these proteins in pairing of centromeric regions and chromosome arms. No homologous pairing of mid-arm or distal regions was observed in rad51, xrcc3, and rad51C mutants. However, homologous centromeres do pair in these mutants and we show that this does depend upon recombination, principally on DMC1. This centromere pairing extends well beyond the heterochromatic centromere region and, surprisingly, does not require XRCC3 and RAD51C. In addition to clarifying and bringing the roles of centromeres in meiotic synapsis to the fore, this analysis thus separates the roles in meiotic synapsis of DMC1 and RAD51 and the meiotic RAD51 paralogs, XRCC3 and RAD51C, with respect to different chromosome domains. Meiosis is a specialised cell division that acts to halve the chromosome complement, or ploidy, in the production of gametes for sexual reproduction in eukaryotes. To ensure that each gamete has a full complement of the genetic material, homologous chromosomes must pair and then separate in a coordinated manner during meiosis, and this is mediated by recombination in the majority of studied eukaryotes. To better understand the relationship between recombination and meiotic homologue pairing, we have analysed meiotic chromosome pairing in plant mutants lacking key recombination proteins. This work provides new insights into the homologous chromosome pairing mechanisms occurring in meiotic prophase of Arabidopsis thaliana: heterochromatic centromeres and 5S rDNA regions pair early, and their pairing has different requirements for recombination proteins than does that of the chromosome arms. These data raise a number of questions concerning the specificities and roles of recombination at different chromosome and/or chromatin regions in the synapsis of homologous chromosomes at meiosis.
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DOI：
10.1073/pnas.0801521105
发表时间：
2008-04-22
影响因子：
11.1
作者：
Colas, Isabelle;Shaw, Peter;Moore, Graham
通讯作者：
Moore, Graham
影响因子：
4.5
作者：
Ho HC;Burgess SM
通讯作者：
Burgess SM
影响因子：
1.6
作者：
CHURCH, K;MOENS, PB
通讯作者：
MOENS, PB
DOI：
10.1083/jcb.200811079
发表时间：
2009-05-18
期刊：
The Journal of cell biology
影响因子：
--
作者：
Badie S;Liao C;Thanasoula M;Barber P;Hill MA;Tarsounas M
通讯作者：
Tarsounas M
影响因子：
27.5
作者：
Boateng, Kingsley A.;Yang, Xiaohui;Makaroff, Christopher A.
通讯作者：
Makaroff, Christopher A.