Surveillance of SARS-CoV-2 in sewage and wastewater treatment plants in Mexico.
Surveillance of SARS-CoV-2 in sewage and wastewater treatment plants in Mexico.
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DOI：
10.1016/j.jwpe.2020.101815
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发表时间：
2021-04
影响因子：
7
通讯作者：
Buitrón G
中科院分区：
文献类型：
--
作者：
Carrillo-Reyes J;Barragán-Trinidad M;Buitrón G
引起COVID-19的SARS-CoV-2病毒通过感染者的粪便在污水中传播，污水中的病毒物质可以使用分子工具进行量化。本研究旨在基于RdRP、S和N基因分析监测墨西哥污水中SARS-CoV-2 RNA的存在。从疫情早期到2020年7月，对两个生活污水处理厂的进水、出水和活性污泥进行了评价。此外，对下水道系统中的采样点进行了检查，比较了两种不同的rna浓缩方法：离心超滤和基于吸附的方法。吸附法的RNA滴定率比超滤法高2个数量级（RdRP基因/mL可达3.38 log10拷贝）。克雷塔罗市两种与COVID-19累积病例相关的污水中SARS-CoV-2 RNA的监测二级污泥中RNA水平高于进水，表明病毒RNA在活性污泥中浓缩。该结果支持在污水处理厂中去除SARS-CoV-2 RNA，其中所有流出样品的病毒定量均为阴性。这项工作证明，在COVID-19诊断检测有限的发展中国家，基于废水的流行病学是一种非常有价值的工具。
The SARS-CoV-2 virus causing COVID-19 is spread in sewage by the stool of infected individuals, and viral material in sewage can be quantified using molecular tools. This study aimed to monitor the presence of SARS-CoV-2 RNA in sewage in Mexico based on RdRP, S, and N gene analysis. The influent, effluent, and activated sludge from two domestic wastewater treatment plants (WWTP) were evaluated from the early stage of the epidemic to July 2020. Additionally, sampling points in sewer systems were examined, comparing two different RNA-concentration methods: centrifugal ultrafiltration and adsorption-based methods. The adsorption method resulted in RNA titration that was two orders of magnitude higher than with ultrafiltration (up to 3.38 log10 copies RdRP gene/mL of sewage). The surveillance of SARS-CoV-2 RNA in the influent of two WWTP correlated with the cumulative COVID-19 cases in Queretaro city. The higher RNA level in secondary sludge compared to influent suggests that viral RNA becomes concentrated in activated sludge. This result supports SARS-CoV-2 RNA removal in WWTP, where all effluent samples were negative for virus quantification. This work proves that wastewater-based epidemiology is a very valuable tool in developing countries where diagnostic tests for COVID-19 are limited.
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作者：
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