Migration of intervertebral disc cells into dense collagen scaffolds intended for functional replacement.
Migration of intervertebral disc cells into dense collagen scaffolds intended for functional replacement.
复制标题
DOI：
10.1007/s10856-011-4545-7
复制
发表时间：
2012-03
影响因子：
3.7
通讯作者：
Smit, Theodoor H.
中科院分区：
文献类型：
--
作者：
Bron, Johannes L.;Mulder, Harriet W.;Vonk, Lucienne A.;Doulabi, Bherouz Zandieh;Oudhoff, M. J.;Smit, Theodoor H.
当使用a细胞支架替代髓核（NP）时，来自周围组织的细胞入侵是再生的关键步骤。本研究的目的是评估NP和周围纤维环（AF）细胞是否能够迁移到致密胶原支架中。我们将新鲜收获的山羊NP和AF细胞植入由1.5%和3.0%的I型胶原基质组成的支架上，通过塑料压缩制备，以评估细胞的侵袭性。4周后，NP细胞的迁移距离（25%）高于AF细胞（10%）。Hst-2（一种从唾液中提取的肽，已知能增强成纤维细胞的迁移）并没有增加迁移距离，这一点在划痕实验中得到了证实。总之，我们揭示了细胞侵入致密胶原支架，从而鼓励了使用a细胞支架替代NP的第一步。
Invasion of cells from surrounding tissues is a crucial step for regeneration when using a-cellular scaffolds as a replacement of the nucleus pulposus (NP). The aim of current study was to assess whether NP and surrounding annulus fibrosus (AF) cells are capable of migrating into dense collagen scaffolds. We seeded freshly harvested caprine NP and AF cells onto scaffolds consisting of 1.5 and 3.0% type I collagen matrices, prepared by plastic compression, to assess cell invasion. The migration distance appeared both time and density dependent and was higher for NP (25%) compared to AF (10%) cells after 4 weeks. Migration distance was not enhanced by Hst-2, a peptide derived from saliva known to enhance fibroblast migration, and this was confirmed in a scratch assay. In conclusion, we revealed invasion of cells into dense collagen scaffolds and therewith encouraging first steps towards the use of a-cellular scaffolds for NP replacement.
登录
查看更多内容
影响因子：
3.4
作者：
Dikovsky, Daniel;Bianco-Peled, Havazelet;Seliktar, Dror
通讯作者：
Seliktar, Dror
DOI：
10.1007/978-1-61779-207-6_2
发表时间：
2011-01-01
期刊：
CELL MIGRATION: DEVELOPMENTAL METHODS AND PROTOCOLS, SECOND EDITION
影响因子：
--
作者：
Cory, Giles
通讯作者：
Cory, Giles
影响因子：
4.9
作者：
Leone, Gemma;Torricelli, Paola;Barbucci, Rolando
通讯作者：
Barbucci, Rolando
影响因子：
2.8
作者：
Bron, Johannes Leendert;Helder, Marco N.;Meisel, Hans-Jorg;Royen, Barend J. Van;Smit, Theodoor H.
通讯作者：
Smit, Theodoor H.
影响因子：
2.8
作者：
Cloyd, Jordan M.;Malhotra, Neil R.;Elliott, Dawn M.
通讯作者：
Elliott, Dawn M.