Swimming under elevated hydrostatic pressure increases glycolytic activity in gas gland cells of the European eel.
Swimming under elevated hydrostatic pressure increases glycolytic activity in gas gland cells of the European eel.
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DOI：
10.1371/journal.pone.0239627
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Pelster B
中科院分区：
文献类型：
--
作者：
Schneebauer G;Lindemann C;Drechsel V;Marohn L;Wysujack K;Santidrian E;Dirks R;Hanel R;Pelster B
尽管经过了几十年的研究，欧洲鳗鲡（Anguilla anguilla）从欧洲海岸到马尾藻海的产卵迁移仍然是一个谜。特别是，鱼鳔作为浮力调节结构的作用还不清楚。在这项研究中，我们行使银鳗鱼在游泳隧道在静水压力升高。然后将这些运动鳗鱼的气腺组织的转录组与未运动（对照）银鳗鱼气腺细胞的已知转录组进行比较。由于鳗鱼种群的高感染率与鱼鳔寄生虫Anguillicola crassus，比较还包括一个运动组的银鳗鱼与严重受损的鱼鳔，我们比较了先前发表的转录组的未行使银鳗鱼与严重受损的鱼鳔行使组的银鳗鱼。未行使（对照）银鳗鱼与行使银鳗鱼功能性的鱼鳔（EF），以及行使银鳗鱼受损的鱼鳔（艾德）的比较，都显示了显着的升高，在转录相关的糖酵解酶。这也可以在具有高度感染的鱼鳔的未锻炼的银鳗鱼与具有受损的鱼鳔（DED）的锻炼鳗鱼的比较中观察到。与EF相反，在艾德中观察到线粒体NADH脱氢酶的转录物数量显著升高。而在EF的转录变化表明，酸的生产和分泌增强，在艾德这些变化似乎与增厚的组织，从而提高扩散距离。显著数量的差异表达的转录本编码的蛋白质连接到cAMP依赖的信号传导途径表明，在气体腺细胞的代谢控制包括cAMP依赖的途径。与艾德相反，EF中显著的转录变化可能与细胞外基质的重建有关，而在艾德中组织修复和炎症更明显。令人惊讶的是，在行使鳗鱼缺氧诱导转录因子的表达升高。在EF中，大量与生物钟相关的基因被转录修饰，这可能与产卵迁移过程中观察到的昼夜垂直迁移有关。
In spite of many decades of research, the spawning migration of the European eel Anguilla anguilla from the European coast to the Sargasso Sea remains a mystery. In particular, the role of the swimbladder as a buoyancy regulating structure is not yet understood. In this study, we exercised silver eels in a swim tunnel under elevated hydrostatic pressure. The transcriptome of gas gland tissue of these exercised eels was then compared to the known transcriptome of not exercised (control) silver eel gas gland cells. Due to the high infection rate of the eel population with the swimbladder parasite Anguillicola crassus, the comparison also included an exercised group of silver eels with a heavily damaged swimbladder, and we compared the previously published transcriptome of not exercised silver eels with a highly damaged swimbladder with the exercised group of silver eels with a heavily damaged swimbladder. The comparisons of unexercised (control) silver eels with exercised silver eels with functional swimbladder (EF), as well as with exercised silver eels with damaged swimbladder (ED), both showed a significant elevation in transcripts related to glycolytic enzymes. This could also be observed within the comparison of unexercised silver eels with a highly infected swimbladder with exercised eels with a damaged swimbladder (DED). In contrast to EF, in ED a significant elevation in transcript numbers of mitochondrial NADH dehydrogenase was observed. While in EF the transcriptional changes suggested that acid production and secretion was enhanced, in ED these changes appeared to be related to thickened tissue and thus elevated diffusion distances. The remarkable number of differentially expressed transcripts coding for proteins connected to cAMP-dependent signaling pathways indicated that metabolic control in gas gland cells includes cAMP-dependent pathways. In contrast to ED, in EF significant transcriptional changes could be related to the reconstruction of the extracellular matrix, while in ED tissue repair and inflammation was more pronounced. Surprisingly, in exercised eels hypoxia inducible transcription factor expression was elevated. In EF, a large number of genes related to the circadian clock were transcriptionally modified, which may be connected to the circadian vertical migrations observed during the spawning migration.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
56.9
作者：
Aarestrup, Kim;Okland, Finn;McKinley, Robert S.
通讯作者：
McKinley, Robert S.
David Gene功能分类工具：一种以生物模块为中心的新型算法，可在功能上分析大基因列表。
影响因子：
12.3
作者：
Huang, Da Wei;Sherman, Brad T;Tan, Qina;Collins, Jack R;Alvord, W Gregory;Roayaei, Jean;Stephens, Robert;Baseler, Michael W;Lane, H Clifford;Lempicki, Richard A
通讯作者：
Lempicki, Richard A
影响因子：
14.9
作者：
B'chir W;Maurin AC;Carraro V;Averous J;Jousse C;Muranishi Y;Parry L;Stepien G;Fafournoux P;Bruhat A
通讯作者：
Bruhat A
AMPK：代谢和线粒体稳态的监护人。
DOI：
10.1038/nrm.2017.95
发表时间：
2018-03
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
Herzig S;Shaw RJ
通讯作者：
Shaw RJ