Regulation of growth and differentiation of a rat hepatoma cell line by the synergistic interactions of hormones and collagenous substrata.
Regulation of growth and differentiation of a rat hepatoma cell line by the synergistic interactions of hormones and collagenous substrata.
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通过激素和胶原基质的协同相互作用调节大鼠肝癌细胞系的生长和分化。
DOI：
10.1083/jcb.97.4.1179
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Reid,LM
中科院分区：
文献类型：
--
作者：
Gatmaitan,Z;Jefferson,DM;Ruiz-Opazo,N;Biempica,L;Arias,IM;Dudas,G;Leinwand,LA;Reid,LM
Serum-free, hormonally defined media have been developed for optimal growth of a rat hepatoma cell line. The cells' hormonal requirements for growth are dramatically altered both qualitatively and quantitatively by whether they were plated onto tissue culture plastic or collagenous substrata. On collagenous substrata, the cells required insulin, glucagon, growth hormone, prolactin, and linoleic acid (bound to BSA), and zinc, copper, and selenium. For growth on tissue culture plastic, the cells required the above factors at higher concentrations plus several additional factors: transferrin, hydrocortisone, and triiodothyronine. To ascertain the relative influence of hormones versus substratum on the growth and differentiation of rat hepatoma cells, various parameters of growth and of liver-specific and housekeeping functions were compared in cells grown in serum-free, hormonally supplemented, or serum-supplemented medium and on either tissue culture plastic or type I collagen gels. The substratum was found to be the primary determinant of attachment and survival of the cells. Even in serum-free media, the cells showed attachment and survival efficiencies of 40-50% at low seeding densities and even higher efficiencies at high seeding densities when the cells were plated onto collagenous substrata. However, optimal attachment and survival efficiencies of the cells on collagenous substrata still required either serum or hormonal supplements. On tissue culture plastic, there was no survival of the cells at any seeding density without either serum or hormonal supplements added to the medium. A defined medium designed for cells plated on tissue culture plastic, containing increased levels of hormones plus additional factors over those in the defined medium designed for cells on collagenous substrata, was found to permit attachment and survival of the cells plated into serum-free medium and onto tissue culture plastic. Growth of the cells was influenced by both substrata and hormones. When plated onto collagen gel substrata as compared with tissue culture plastic, the cells required fewer hormones and growth factors in the serum-free, hormone-supplemented media to achieve optimal growth rates. Growth rates of the cells at low and high seeding densities were equivalent in the hormonally and serum-supplemented media as long as comparisons were made on the same substratum and the hormonally supplemented medium used was the one designed for that substratum. For a given medium, either serum or hormonally supplemented, the saturation densities were highest for tissue culture plastic as compared with collagen gels.(ABSTRACT TRUNCATED AT 400 WORDS)
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生长激素可能控制大鼠肝脏谷氨酰胺合成酶活性。
影响因子：
4.8
作者：
B. S. Wong;M. Chenoweth;A. Dunn
通讯作者：
A. Dunn
胰高血糖素和胰岛素对原代组织培养中新生大鼠肝细胞生长的影响。
影响因子：
4.8
作者：
U. Armato;E. Draghi;P. Andreis
通讯作者：
P. Andreis
非转化细胞的生存和生长要求
DOI：
--
发表时间：
1981
期刊：
影响因子：
--
作者：
R. Ham
通讯作者：
R. Ham
DOI：
10.1093/jn/91.4.473
发表时间：
1967
期刊：
The Journal of nutrition
影响因子：
--
作者：
D. V. Campen;Priscilla U. Scaife
通讯作者：
Priscilla U. Scaife
细胞形状和表面结构中的微丝-膜相互作用
DOI：
--
发表时间：
1981
期刊：
影响因子：
--
作者：
J. Condeelis
通讯作者：
J. Condeelis