Nanopolystyrene translocation and fetal deposition after acute lung exposure during late-stage pregnancy.
Nanopolystyrene translocation and fetal deposition after acute lung exposure during late-stage pregnancy.
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妊娠晚期急性肺暴露后纳米聚苯乙烯移位与胎儿沉积。
DOI：
10.1186/s12989-020-00385-9
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发表时间：
2020-10-24
影响因子：
10
通讯作者：
Stapleton PA
中科院分区：
文献类型：
--
作者：
Fournier SB;D'Errico JN;Adler DS;Kollontzi S;Goedken MJ;Fabris L;Yurkow EJ;Stapleton PA
塑料无处不在。它用于食品包装、存储容器、电子产品、家具、服装和常见的一次性物品。微塑料和纳米塑料微粒是由塑料污染的大块破碎和解体形成的。最近在室内空气和遥远的大气沉降物中发现了塑料微粒。由于其体积小，大气中的微塑料和纳米塑料颗粒可被吸入，并可能对人类健康构成风险，特别是对易感人群。当吸入时，纳米颗粒已被证明可以越过肺细胞屏障转移到次要器官，包括胎盘。然而，纳米塑料颗粒母婴易位的可能性以及纳米塑料沉积或积聚对胎儿健康的影响尚不清楚。在这项研究中，我们研究了纳米聚苯乙烯颗粒是否可以穿过胎盘屏障并在母体肺部暴露后沉积在胎儿组织中。妊娠大鼠于妊娠第19天（GD）气管内注射20 nm罗丹明标记的纳米聚苯乙烯微球（2.64 × 1014粒）。GD 20 24小时后，使用荧光光学成像评估母体和胎儿组织。固定胎儿组织，用高光谱显微镜观察颗粒。利用离体胎盘灌注，将已知浓度的纳米聚苯乙烯注入子宫动脉。收集母体和胎儿流出物180分钟，评估聚苯乙烯颗粒浓度。与对照组相比，母亲接触24小时后，胎儿和胎盘重量显著降低（分别为7%和8%）。在母体肺、心、脾中检测到纳米聚苯乙烯颗粒。在胎盘、胎儿肝脏、肺、心脏、肾脏和大脑中也观察到聚苯乙烯纳米颗粒，这表明在妊娠晚期母体肺到胎儿组织的纳米颗粒易位。这些研究证实，母体肺部接触纳米聚苯乙烯会导致塑料颗粒易位到胎盘和胎儿组织，并使胎儿胎盘单位容易受到不利影响。这些数据对于了解塑料微粒毒理学以及健康和疾病的发展起源至关重要。
Plastic is everywhere. It is used in food packaging, storage containers, electronics, furniture, clothing, and common single-use disposable items. Microplastic and nanoplastic particulates are formed from bulk fragmentation and disintegration of plastic pollution. Plastic particulates have recently been detected in indoor air and remote atmospheric fallout. Due to their small size, microplastic and nanoplastic particulate in the atmosphere can be inhaled and may pose a risk for human health, specifically in susceptible populations. When inhaled, nanosized particles have been shown to translocate across pulmonary cell barriers to secondary organs, including the placenta. However, the potential for maternal-to-fetal translocation of nanosized-plastic particles and the impact of nanoplastic deposition or accumulation on fetal health remain unknown. In this study we investigated whether nanopolystyrene particles can cross the placental barrier and deposit in fetal tissues after maternal pulmonary exposure. Pregnant Sprague Dawley rats were exposed to 20 nm rhodamine-labeled nanopolystyrene beads (2.64 × 1014 particles) via intratracheal instillation on gestational day (GD) 19. Twenty-four hours later on GD 20, maternal and fetal tissues were evaluated using fluorescent optical imaging. Fetal tissues were fixed for particle visualization with hyperspectral microscopy. Using isolated placental perfusion, a known concentration of nanopolystyrene was injected into the uterine artery. Maternal and fetal effluents were collected for 180 min and assessed for polystyrene particle concentration. Twenty-four hours after maternal exposure, fetal and placental weights were significantly lower (7 and 8%, respectively) compared with controls. Nanopolystyrene particles were detected in the maternal lung, heart, and spleen. Polystyrene nanoparticles were also observed in the placenta, fetal liver, lungs, heart, kidney, and brain suggesting maternal lung-to-fetal tissue nanoparticle translocation in late stage pregnancy. These studies confirm that maternal pulmonary exposure to nanopolystyrene results in the translocation of plastic particles to placental and fetal tissues and renders the fetoplacental unit vulnerable to adverse effects. These data are vital to the understanding of plastic particulate toxicology and the developmental origins of health and disease.
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作者：
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DOI：
10.1152/ajpheart.00634.2016
发表时间：
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影响因子：
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作者：
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