Tibetan Medical Formula Shi-Wei-Gan-Ning-Pill Protects Against Carbon Tetrachloride-Induced Liver Fibrosis - An NMR-Based Metabolic Profiling.
Tibetan Medical Formula Shi-Wei-Gan-Ning-Pill Protects Against Carbon Tetrachloride-Induced Liver Fibrosis - An NMR-Based Metabolic Profiling.
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DOI：
10.3389/fphar.2018.00965
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发表时间：
2018
影响因子：
5.6
通讯作者：
Zhong GJ
中科院分区：
文献类型：
--
作者：
Feng X;Li MH;Xia J;Deng Ba DJ;Ruan LY;Xing YX;Chen C;Wang JS;Zhong GJ
肝纤维化是一个严重的健康问题，威胁生命质量并导致死亡，引起全世界的高度关注。十味甘宁丸（SWGNP）是一种传统的藏方，用于治疗肝损伤；但其保肝机制尚未完全阐明。在本研究中，应用组织学染色、生化检测和元素测定来评估SWGNP对四氯化碳（CCl4）诱导的肝纤维化大鼠模型的抗纤维化功效。基于 NMR 的代谢组学结合正交偏最小二乘判别分析 (OPLS-DA)、典型回归分析和相关网络分析用于表征潜在的生物标志物以及与 SWGNP 保肝活性相关的代谢途径。结果显示，SWGNP可以显着减轻病理变化，降低纤维化标志物（ColIV、HA、LN和PCIII）的水平，并调节紊乱元素的分布。多变量分析和相关网络分析表明，SWGNP可以通过抗氧化、修复受损的能量代谢以及逆转紊乱的氨基酸和核酸代谢来保护大鼠免受CCl4诱导的肝纤维化。总之，这种综合代谢组学方法为 SWGNP 在肝纤维化疾病中的保肝作用机制提供了新的见解。
Liver fibrosis is a severe health problem, threatening the life quality and causing death, raising great concerns worldwide. Shi-Wei-Gan-Ning-Pill (SWGNP) is a traditional Tibetan recipe used to treat hepatic injuries; however, its hepatoprotective mechanism has not yet fully clarified. In this study, histological staining, biochemical assays, and elements determination were applied to evaluate the anti-fibrotic efficacy of SWGNP on a carbon tetrachloride (CCl4) induced hepato-fibrosis rat model. NMR-based metabolomics combined with orthogonal partial least squares-discriminant analysis (OPLS-DA), canonical regression analysis, and correlation networks analysis was used to characterize the potential biomarkers as well as metabolic pathways associated with the hepatoprotective activity of SWGNP. The results showed that SWGNP could significantly attenuate the pathological changes and decrease the levels of fibrosis markers (ColIV, HA, LN, and PCIII), and regulate the disordered elements distribution. Multivariate analysis and correlation network analysis revealed that SWGNP could protect rats against CCl4-induced liver fibrosis through anti-oxidation, repairing the impaired energy metabolisms and reversing the disturbed amino acids and nucleic acids metabolisms. In conclusion, this integrated metabolomics approach provided new insights into the mechanism of the hepatoprotective effect of SWGNP in liver fibrosis disease.
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