Primary sequence analysis by fast atom bombardment mass spectrometry of a peptide with adipokinetic activity from the corpora cardiaca of the cricket Gryllus bimaculatus.

Primary sequence analysis by fast atom bombardment mass spectrometry of a peptide with adipokinetic activity from the corpora cardiaca of the cricket Gryllus bimaculatus.
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通过快原子轰击质谱法对来自蟋蟀双斑蝼蛄心脏体的具有脂肪运动活性的肽进行一级序列分析。

DOI:
10.1016/0006-291x(87)90494-3
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发表时间:
1987
影响因子:
3.1
通讯作者:
Rinehart,KL
Rinehart,KL
中科院分区:
生物学4区
文献类型:
--
作者:
Gäde,G;Rinehart,KL

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从蟋蟀Gryllus bimaculatus中分离的八肽AKH-G刺激蟋蟀本身和蝗虫中的脂质动员。AKH G的结构经快原子轰击质谱(FABMS)鉴定为pGlu Val Asn Phe Ser Thr Gly Trp NH 2,与血吸虫AKH Ⅱ S的结构最为接近,后者具有Leu 2结构单元。固相法合成的AKH-G具有与天然物质相同的生物活性。FABMS测量,包括高分辨率和亚稳的研究,采用400-900 pmole的样品。
The octapeptide AKH-G, isolated from the cricket Gryllus bimaculatus, stimulates lipid mobilization in both the cricket itself and in migratory locusts. The structure of AKH-G has been assigned by fast atom bombardment mass spectrometry (FABMS) as pGlu-Val-Asn-Phe-Ser-Thr-Gly-Trp-NH 2, and it is most closely related to AKH II-S from Schistocerca species, which has a Leu 2 unit. Synthetic AKH-G, prepared by solid phase techniques, had the same bioactivity as the natural substance. FABMS measurements, including high-resolution and metastable studies, were made employing 400–900 pmole of sample.
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DOI: 10.1016/0022-1910(86)90003-x
发表时间: 1986
影响因子: 2.2
作者:
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通讯作者: G. Gäde
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发表时间: 1980-01-01
影响因子: 2.2
作者:
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发表时间: 1984-01-01
影响因子: --
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影响因子: --
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通讯作者: C. Wheeler
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发表时间: 1986
影响因子: 3.1
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