Annexin5 plays a vital role in Arabidopsis pollen development via Ca2+-dependent membrane trafficking.
Annexin5 plays a vital role in Arabidopsis pollen development via Ca2+-dependent membrane trafficking.
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Annexin5 通过 Ca2 依赖性膜运输在拟南芥花粉发育中发挥重要作用
DOI：
10.1371/journal.pone.0102407
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Xiang Y
中科院分区：
文献类型：
--
作者：
Zhu J;Wu X;Yuan S;Qian D;Nan Q;An L;Xiang Y
花粉发育和花粉管的调节是一个复杂的生物学过程，对于开花植物的有性繁殖至关重要。拟南芥5（ANN5）在转基因ANN5-RNAI线中的功能下调，对ANN5在花粉中的功能的基础机制很少知道。发芽花粉管的生长和过表达ANN 5的花粉管的耐药性增加，而钙的过度表达了Ca2+依赖于latb firctination for lactrund的钙的钙促进了Ca2+的细胞质量。优先定位动态actin filament arrays and preventing actin polymerization. Overexpression of Ann5 did not affect pollen germination or pollen tube growth in response to LatB compared with wild-type, although Ann5 interacts with actin filaments in a manner similar to some animal annexins. In addition, the sterile pollen phenotype could be only partially responded by Ann5 mutants at Ca2+-binding sites when compared to the complete recovery by wild-type Ann5. These data demonstrated ANN5通过钙调制了我们的钙的内膜运输调节，参与花粉的发育，发芽和花粉管的生长。
The regulation of pollen development and pollen tube growth is a complicated biological process that is crucial for sexual reproduction in flowering plants. Annexins are widely distributed from protists to higher eukaryotes and play multiple roles in numerous cellular events by acting as a putative “linker” between Ca2+ signaling, the actin cytoskeleton and the membrane, which are required for pollen development and pollen tube growth. Our recent report suggested that downregulation of the function of Arabidopsis annexin 5 (Ann5) in transgenic Ann5-RNAi lines caused severely sterile pollen grains. However, little is known about the underlying mechanisms of the function of Ann5 in pollen. This study demonstrated that Ann5 associates with phospholipid membrane and this association is stimulated by Ca2+ in vitro. Brefeldin A (BFA) interferes with endomembrane trafficking and inhibits pollen germination and pollen tube growth. Both pollen germination and pollen tube growth of Ann5-overexpressing plants showed increased resistance to BFA treatment, and this effect was regulated by calcium. Overexpression of Ann5 promoted Ca2+-dependent cytoplasmic streaming in pollen tubes in vivo in response to BFA. Lactrunculin (LatB) significantly prohibited pollen germination and tube growth by binding with high affinity to monomeric actin and preferentially targeting dynamic actin filament arrays and preventing actin polymerization. Overexpression of Ann5 did not affect pollen germination or pollen tube growth in response to LatB compared with wild-type, although Ann5 interacts with actin filaments in a manner similar to some animal annexins. In addition, the sterile pollen phenotype could be only partially rescued by Ann5 mutants at Ca2+-binding sites when compared to the complete recovery by wild-type Ann5. These data demonstrated that Ann5 is involved in pollen development, germination and pollen tube growth through the promotion of endomembrane trafficking modulated by calcium. Our results provide reliable molecular mechanisms that underlie the function of Ann5 in pollen.
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影响因子：
4.9
作者：
Breton, G;Vazquez-Tello, A;Sarhan, F
通讯作者：
Sarhan, F
影响因子：
6.9
作者：
Bai S;Willard B;Chapin LJ;Kinter MT;Francis DM;Stead AD;Jones ML
通讯作者：
Jones ML
DOI：
10.1093/bioinformatics/btq662
发表时间：
2011-02-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Benkert P;Biasini M;Schwede T
通讯作者：
Schwede T
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
5.6
作者：
Lizarbe MA;Barrasa JI;Olmo N;Gavilanes F;Turnay J
通讯作者：
Turnay J