Repair of segmental load-bearing bone defect by autologous mesenchymal stem cells and plasma-derived fibrin impregnated ceramic block results in early recovery of limb function.
Repair of segmental load-bearing bone defect by autologous mesenchymal stem cells and plasma-derived fibrin impregnated ceramic block results in early recovery of limb function.
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DOI：
10.1155/2014/345910
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发表时间：
2014
影响因子：
--
通讯作者：
Idrus RB
中科院分区：
文献类型：
--
作者：
Ng MH;Duski S;Tan KK;Yusof MR;Low KC;Rose IM;Mohamed Z;Bin Saim A;Idrus RB
磷酸钙基骨替代材料由于力学性能差，尚未用于修复承重骨缺损。在这项研究中，我们重新评估了陶瓷块与成骨诱导的间充质干细胞和富血小板血浆（TEB）联合修复关键尺寸节段性胫骨缺损的功能结果。将其与新鲜骨髓陶瓷块（MIC）和部分脱矿同种异体骨块（ALLO）进行比较。每个研究组使用6只新西兰白色雌性家兔，3只无植入物的家兔用作阴性对照。到第90天，TEB组中4/6只兔以及ALLO和MIC组中2/6只兔恢复正常步态模式。TEB组的愈合速度明显更快，放射学评分为4.50 ± 0.78，而ALLO组（1.06 ± 0.32）、MIC组（1.28 ± 0.24）和阴性对照组（0）的愈合速度明显更快。组织学上，TEB组的新骨百分比最高（82% ± 5.1%），而ALLO组（5% ± 2.5%）和MIC组（26% ± 5.2%）。在生物力学方面，TEB处理的胫骨达到了最高的压缩强度（43.50 ± 12.72 MPa），而ALLO（15.15 ± 3.57 MPa）和MIC（23.28 ± 6.14 MPa）处理的胫骨达到了最高的压缩强度。总之，TEB可以修复临界尺寸的节段性承重骨缺损，恢复肢体功能。
Calcium phosphate-based bone substitutes have not been used to repair load-bearing bone defects due to their weak mechanical property. In this study, we reevaluated the functional outcomes of combining ceramic block with osteogenic-induced mesenchymal stem cells and platelet-rich plasma (TEB) to repair critical-sized segmental tibial defect. Comparisons were made with fresh marrow-impregnated ceramic block (MIC) and partially demineralized allogeneic bone block (ALLO). Six New Zealand White female rabbits were used in each study group and three rabbits with no implants were used as negative controls. By Day 90, 4/6 rabbits in TEB group and 2/6 in ALLO and MIC groups resumed normal gait pattern. Union was achieved significantly faster in TEB group with a radiological score of 4.50 ± 0.78 versus ALLO (1.06 ± 0.32), MIC (1.28 ± 0.24), and negative controls (0). Histologically, TEB group scored the highest percentage of new bone (82% ± 5.1%) compared to ALLO (5% ± 2.5%) and MIC (26% ± 5.2%). Biomechanically, TEB-treated tibiae achieved the highest compressive strength (43.50 ± 12.72 MPa) compared to those treated with ALLO (15.15 ± 3.57 MPa) and MIC (23.28 ± 6.14 MPa). In conclusion, TEB can repair critical-sized segmental load-bearing bone defects and restore limb function.
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影响因子：
1.9
作者：
Pieri, Francesco;Lucarelli, Enrico;Marchetti, Claudio
通讯作者：
Marchetti, Claudio
影响因子：
5.3
作者：
Boos AM;Loew JS;Deschler G;Arkudas A;Bleiziffer O;Gulle H;Dragu A;Kneser U;Horch RE;Beier JP
通讯作者：
Beier JP
影响因子：
5.4
作者：
Fan, Zhao-Xin;Lu, Yao;Xie, Hui-Qi
通讯作者：
Xie, Hui-Qi
影响因子：
1.7
作者：
Ng, AMH;Bin Saim, A;Idrus, RBH
通讯作者：
Idrus, RBH
影响因子：
4.1
作者：
Liu, Guangpeng;Li, Yulin;Cao, Yilin
通讯作者：
Cao, Yilin