The pattern of c-Fos expression and its refractory period in the brain of rats and monkeys.
The pattern of c-Fos expression and its refractory period in the brain of rats and monkeys.
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DOI：
10.3389/fncel.2015.00072
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发表时间：
2015
影响因子：
5.3
通讯作者：
Mello LE
中科院分区：
文献类型：
--
作者：
Barros VN;Mundim M;Galindo LT;Bittencourt S;Porcionatto M;Mello LE
神经元的强烈激活触发立即表达基因的出现，包括c-Fos。该基因与参与生物化学过程的各种信号级联相关，例如神经元可塑性、细胞生长和有丝分裂。本研究观察了戊四氮（pentylenetrazol）刺激后大鼠和猴脑内c-Fos的表达模式和不应期。在PTZ诱导癫痫发作后的不同时间处死大鼠和猴。在这里，我们发现，大鼠和猴子已经显示c-Fos表达在0.5小时后癫痫发作。然而，猴子中的蛋白质表达模式比大鼠长，并且与大鼠相反，猴子不同脑区的蛋白质表达模式也不均匀（相对强度）。此外，猴子的c-Fos表达的时间分布方面的区域性脑变化，这是没有看到在大鼠。第二次PTZ刺激后的不应期在大鼠和猴子之间也有显着差异，后者甚至在第二次刺激后对c-Fos表达表现出总结性作用。然而，大鼠c-Fos mRNA的评估表明，转录后控制机制的持续时间的不应期。啮齿类动物和灵长类动物中蛋白质表达模式的差异表征了这些哺乳动物之间不同的脑生物化学的功能方面，并且与给予c-Fos参与认知和学习任务的啮齿类动物相比，可能有助于更发达的灵长类动物认知复杂性。
Intense activation of neurons triggers the appearance of immediate expression genes, including c-Fos. This gene is related to various signal cascades involved in biochemical processes such as neuronal plasticity, cell growth and mitosis. Here we investigate the expression pattern and the refractory period of c-Fos in rats and monkey’s brains after stimulation with pentylenetetrazol. Rats and monkeys were sacrificed at various times after PTZ-induced seizure. Here we show that rats and monkeys already showed c-Fos expression at 0.5 h after seizure. Yet, the pattern of protein expression was longer in monkeys than rats, and also was not uniform (relative intensity) across different brain regions in monkeys as opposed to rats. In addition monkeys had a regional brain variation with regard to the temporal profile of c-Fos expression, which was not seen in rats. The refractory period after a second PTZ stimulation was also markedly different between rats and monkeys with the latter even showing a summatory effect on c-Fos expression after a second stimulation. However, assessment of c-Fos mRNA in rats indicated a post-transcriptional control mechanism underlying the duration of the refractory period. The difference in the protein expression pattern in rodents and primates characterizes a functional aspect of brain biochemistry that differs between these mammalian orders and may contribute for the more developed primate cognitive complexity as compared to rodents given c-Fos involvement in cognitive and learning tasks.
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