Chemical mechanism of the adenosine cyclic 3',5'-monophosphate dependent protein kinase from pH studies.

Chemical mechanism of the adenosine cyclic 3',5'-monophosphate dependent protein kinase from pH studies.
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pH 研究中腺苷环 3,5-单磷酸依赖性蛋白激酶的化学​​机制。

DOI:
10.1021/bi00387a056
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发表时间:
1987
期刊:
影响因子:
2.9
通讯作者:
Cook,PF
Cook,PF
中科院分区:
生物学3区
文献类型:
--
作者:
Yoon,MY;Cook,PF

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Materials and MethodsChemicals. Phosphoenolpyruvate, ATP, NADH, Mes, Hepes, Mops, Taps, BTP, and AMP-PCP were from Sigma. Gold label (100 atom% D) D20 was from Aldrich. All peptides were prepared by using the solid-phase synthesis of1 Abbreviations: BTP, l-[bis (hydroxymethyl) amino]-3-tris (hydroxymethy!) propane; Mes, Z-CN-morpholinolethanesulfonic acid; Mops, 4-morpholinepropanesulfonic acid; Taps, 3-[[tris (hydroxymethyl) methyl]-amino] propanesulfonic acid; PEP, phosphoenolpyruvate; FSBA, 5'-[p-(fluorosulfonyl) benzoyl] adenosine; AMP-PCP, 5'-adenylyl methylenediphosphate; cA protein kinase, adenosine cyclic 3', 5'-monophosphate dependent protein kinase; Mgf2+, the uncomplexed form of Mg2+, that is, corrected for chelate complex formation; Ser-peptide, Leu-Arg-Arg-Ala-Ser-Leu-Gly; Ala-peptide, Leu-Arg-Arg-Ala-Ala-Leu-Gly; HPLC, high-performance liquid chromatography; ATP, adenosine 5'-tri-phosphate; GTP, guanosine 5'-triphosphate; 1TP, inosine 5'-triphosphate; ADP, adenosine 5'-diphosphate; NADH, reduced nicotinamide adenine dinucleotide.
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DOI: 10.1021/bi00333a024
发表时间: 1985
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