The Deletion of LeuRS Revealed Its Important Roles in Osmotic Stress Tolerance, Amino Acid and Sugar Metabolism, and the Reproduction Process of Aspergillus montevidensis.
The Deletion of LeuRS Revealed Its Important Roles in Osmotic Stress Tolerance, Amino Acid and Sugar Metabolism, and the Reproduction Process of Aspergillus montevidensis.
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DOI：
10.3390/jof10010036
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发表时间：
2024-01-03
期刊：
影响因子：
4.7
通讯作者：
Liu, Yue
中科院分区：
文献类型：
--
作者：
Ding, Xiaowei;Liu, Wanting;Liu, Kaihui;Gao, Xiang;Liu, Yue
蒙特维德曲霉是一种重要的驯化真菌，在高渗条件下已被应用于许多传统发酵食品的生产。然而，渗透胁迫耐受性的详细机制仍不清楚。在此，我们构建了A.在盐胁迫和非盐胁迫条件下，ΔLeuRS突变体生长缓慢，并且与野生型菌株（WT）相比抑制闭囊壳的发育。此外，LeuRS调控的差异表达基因（p < 0.001）涉及耐盐性、ABC转运蛋白、氨基酸代谢、糖代谢和生殖过程。与WT菌株相比，在短期和长期盐胁迫下，ΔLeuRS菌株特别改变了代谢物（例如氨基酸及其衍生物、碳水化合物、有机酸和脂肪酸）的积累水平。本研究为深入了解嗜盐菌耐盐机理提供了新的思路，为嗜盐菌发酵食品风味改良奠定了基础。蒙特维多西斯。
Aspergillus montevidensis is an important domesticated fungus that has been applied to produce many traditional fermented foods under high osmotic conditions. However, the detailed mechanisms of tolerance to osmotic stress remain largely unknown. Here, we construct a target-deleted strain (ΔLeuRS) of A. montevidensis and found that the ΔLeuRS mutants grew slowly and suppressed the development of the cleistothecium compared to the wide-type strains (WT) under salt-stressed and non-stressed conditions. Furthermore, differentially expressed genes (p < 0.001) governed by LeuRS were involved in salt tolerance, ABC transporter, amino acid metabolism, sugar metabolism, and the reproduction process. The ΔLeuRS strains compared to WT strains under short- and long-term salinity stress especially altered accumulation levels of metabolites, such as amino acids and derivatives, carbohydrates, organic acids, and fatty acids. This study provides new insights into the underlying mechanisms of salinity tolerance and lays a foundation for flavor improvement of foods fermented with A. montevidensis.
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影响因子：
16.5
作者：
Krijgsheld P;Bleichrodt R;van Veluw GJ;Wang F;Müller WH;Dijksterhuis J;Wösten HA
通讯作者：
Wösten HA
影响因子：
5.1
作者：
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通讯作者：
Shinozaki, K
影响因子：
5
作者：
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通讯作者：
Gong, Guoli
DOI：
10.1073/pnas.1801287115
发表时间：
2018-06-05
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
14.9
作者：
Ji QQ;Fang ZP;Ye Q;Chi CW;Wang ED
通讯作者：
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