Bulked Segregant RNA-Seq Reveals Distinct Expression Profiling in Chinese Wheat Cultivar Jimai 23 Responding to Powdery Mildew
Bulked Segregant RNA-Seq Reveals Distinct Expression Profiling in Chinese Wheat Cultivar Jimai 23 Responding to Powdery Mildew
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批量分离 RNA 测序揭示了中国小麦品种济麦 23 对白粉病的独特表达谱
DOI：
10.3389/fgene.2020.00474
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发表时间：
2020-05
影响因子：
3.7
通讯作者：
Ma Pengtao
中科院分区：
文献类型：
--
作者：
Zhu Tong;Wu Liru;He Huagang;Song Jiancheng;Jia Mengshu;Liu Liancheng;Wang Xiaolu;Han Ran;Niu Liping;Du Wenxiao;Zhang Xu;Wang Wenrui;Liang Xiao;Li Haosheng;Liu Jianjun;Xu Hongxing;Liu Cheng;Ma Pengtao
小麦白粉病是由小麦白粉病菌Blumeria graminis f.小麦赤霉病（Bgt）是威胁全球小麦生产的最具破坏性的真菌病害之一。寄主抗性是控制该病最有效的方法。然而，小麦抗白粉病的分子机制尚不清楚。为了分析Pm的分子机制，我们以抗白粉病小麦品种济麦23为材料，研究了Pm的潜在抗性组分，并利用混合分离体RNA-Seq（BSR-Seq）分析了Pm在白粉病侵染过程中的表达。结果表明，济麦23的Pm由一对显性基因控制，暂命名为PmJM 23，并将其定位于第5DS染色体上的Pm 2区域。在抗感亲本和抗感亲本济麦23与感病亲本台农18的组合中，共检测到3,816个一致性不同的SNPs。其中58个SNP被分配到PmJM 23的候选区域。随后，通过GO、COG和KEGG途径富集，分析了亲本和原液之间的3，803个差异表达基因（DEG）。通过RT-qPCR进一步确定Bgt接种后相关基因的时间表达模式。研究了6个抗病相关基因在Bgt感染过程中的表达，为Bgt持久抗性的改良提供了宝贵的遗传资源。
Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is one of the most destructive fungal diseases threatening global wheat production. Host resistance is well known to be the most efficient method to control this disease. However, the molecular mechanism of wheat powdery mildew resistance (Pm) is still unclear. To analyze the molecular mechanism of Pm, we used the resistant wheat cultivar Jimai 23 to investigate its potential resistance components and profiled its expression in response to powdery mildew infection using bulked segregant RNA-Seq (BSR-Seq). We showed that the Pm of Jimai 23 was provided by a single dominant gene, tentatively designated PmJM23, and assigned it to the documented Pm2 region of chromosome 5DS. 3,816 consistently different SNPs were called between resistant and susceptible parents and the bulked pools derived from the combinations between the resistant parent Jimai23 and the susceptible parent Tainong18. 58 of the SNPs were assigned to the candidate region of PmJM23. Subsequently, 3,803 differentially expressed genes (DEGs) between parents and bulks were analyzed by GO, COG and KEGG pathway enrichment. The temporal expression patterns of associated genes following Bgt inoculation were further determined by RT-qPCR. Expression of six disease-related genes was investigated during Bgt infection and might serve as valuable genetic resources for the improvement of durable resistance to Bgt.
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DOI：
10.1007/978-981-13-9853-7_7
发表时间：
2019
期刊：
Powdery Mildew Disease of Crucifers: Biology, Ecology and Disease Management
影响因子：
--
作者：
G. S. Saharan;N. Mehta;P. Meena
通讯作者：
G. S. Saharan;N. Mehta;P. Meena
批量分类RNA-Seq揭示了针对cat鱼肠败血症的抗病性抗病性的表达和位置候选基因和等位基因特异性表达。
影响因子：
4.4
作者：
Wang R;Sun L;Bao L;Zhang J;Jiang Y;Yao J;Song L;Feng J;Liu S;Liu Z
通讯作者：
Liu Z
DOI：
--
发表时间：
2000
期刊：
Wheat Information Service
影响因子：
--
作者：
J. Dubcovsky;C. Morris;R. Appels;Cic-Biolab Azul;M. Francki;Paul Murphy
通讯作者：
J. Dubcovsky;C. Morris;R. Appels;Cic-Biolab Azul;M. Francki;Paul Murphy
影响因子：
5.4
作者：
Yong Wang;Jingzhong Xie;Huaizhi Zhang;B. Guo;S. Ning;Yongxing Chen;P. Lu;Qiuhong Wu;Miaomiao Li-M
通讯作者：
Yong Wang;Jingzhong Xie;Huaizhi Zhang;B. Guo;S. Ning;Yongxing Chen;P. Lu;Qiuhong Wu;Miaomiao Li-M
DOI：
10.1073/pnas.0606330103
发表时间：
2006-11-14
影响因子：
11.1
作者：
Fauteux, Francois;Chain, Florian;Belanger, Richard R.
通讯作者：
Belanger, Richard R.