The roles of ascorbic acid and glutathione in symptom alleviation to SA-deficient plants infected with RNA viruses

The roles of ascorbic acid and glutathione in symptom alleviation to SA-deficient plants infected with RNA viruses
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抗坏血酸和谷胱甘肽在缓解 RNA 病毒感染的 SA 缺陷植物症状中的作用

DOI:
10.1007/s00425-011-1391-2
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发表时间:
2011-03
期刊:
影响因子:
4.3
通讯作者:
Zhang, Zhong-Wei
Zhang, Zhong-Wei
中科院分区:
生物学2区
文献类型:
--
作者:
Zhu, Feng;Shang, Jing;Xi, De-Hui;Wang, Shao-Dong;Jia, Shu-Dan;Xu, Fei;Wang, Jian-Hui;Yuan, Shu;Xu, Mo-Yun;Yang, Hui;Lin, Hong-Hui;Zhang, Zhong-Wei

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水杨酸(SA)是植物系统获得性病毒抗性(SAR)所必需的。而缺乏sa的植株对RNA病毒感染的适应能力较强,症状较轻,活性氧积累较少。在前10天内,sa缺乏植株的病毒复制水平较高,但在20 dpi后低于野生型幼苗。谷胱甘肽和抗坏血酸(AsA)水平的升高可能是其症状减轻的原因之一。对于致命病毒,单独的病毒控制方法导致坏死和褪绿,无论病毒rna的积累水平如何。相比之下,早期和高剂量AsA治疗可缓解症状,并在20天后最终抑制病毒复制。ROS消除剂不能模仿AsA的作用,既不能缓解症状,也不能抑制病毒复制。提示植物病毒治疗应兼顾缓解症状和控制病毒复制。
Salicylic acid (SA) is required for plant systemic acquired resistance (SAR) to viruses. However, SA-deficient plants adapt to RNA virus infections better, which show a lighter symptom and have less reactive oxygen species (ROS) accumulation. The virus replication levels are higher in the SA-deficient plants during the first 10 days, but lower than the wild-type seedlings after 20 dpi. The higher level of glutathione and ascorbic acid (AsA) in SA-deficient plants may contribute to their alleviated symptoms. Solo virus-control method for mortal viruses results in necrosis and chlorosis, no matter what level of virus RNAs would accumulate. Contrastingly, early and high-dose AsA treatment alleviates the symptom, and eventually inhibits virus replication after 20 days. ROS eliminators could not imitate the effect of AsA, and could neither alleviate symptom nor inhibit virus replication. It suggests that both symptom alleviation and virus replication control should be considered for plant virus cures.
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