Valorization of chicken slaughterhouse by-products: Production and properties of chicken trachea hydrolysates using commercial proteases
Valorization of chicken slaughterhouse by-products: Production and properties of chicken trachea hydrolysates using commercial proteases
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鸡屠宰场副产品的增值：使用商业蛋白酶生产鸡气管水解产物和特性
DOI：
10.1080/10942912.2021.1986522
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发表时间：
2021
影响因子：
2.9
通讯作者：
Pramualkijja T
中科院分区：
文献类型：
--
作者：
Pramualkijja T
胶原蛋白是一种丰富的结构蛋白，存在于动物的许多器官中，例如皮肤、骨骼和结缔组织。鸡气管是鸡内脏中富含胶原蛋白的部分，在家禽加工过程中转化为固体废物流。胶原蛋白水解产物和多肽具有独特的生物学特性，有潜力从鸡气管中转化为有用的功能性成分。新鲜鸡气管经121℃高压灭菌30 min后，用冷冻干燥机干燥得到鸡气管薄片（CTF）。CTF蛋白含量为68.56%，pH值为6 ~ 10时溶解度为50%。采用Alcalase®、Flavourzyme®、Protamex®和木瓜蛋白酶（Papain）对鸡气管进行酶解，各酶在最佳条件下进行酶解。观察到溶解度的改善。在Alcalase®水解的样品中检测到溶解度最高的鸡气管水解物（CTH），其次分别是Protamex®、Papain和Flavourzyme®。在水解1小时内，从Alcalase®水解获得的CTH具有最高的总氨基酸含量。在这种水解粉末中发现的前三种氨基酸是谷氨酸（Glu）、甘氨酸（Gly）和天冬氨酸（Asp）。DPPH和FRAP实验显示，该样品抗氧化能力最高（分别为4.42 TEACµMol/mg CTH和22.48 TEACµMol/mg CTH）， ACE I抑制剂ic50最低（0.41 mg/mL），具有较好的抗氧化和生物活性。这些结果表明，Alcalase®水解为鸡气管胶原蛋白水解产物提供了独特的性能，可用于各种食品体系。
Collagen is an abundant structural protein found in many organs of animals, for example skin, bones, and connective tissues. Chicken trachea is a collagen-rich fraction of chicken offal that is converted to a solid waste stream during poultry processing. Collagen hydrolysates and peptides have unique biological properties, which is potentially converted from chicken trachea and can be used as a useful functional ingredient. Fresh chicken trachea was sterilized by autoclaving at 121°C for 30 min and dried using freeze drier to obtain chicken trachea flakes (CTF). CTF had 68.56% protein with the 50% solubility at pH 6–10. The enzymatic hydrolysis of chicken trachea was performed using Alcalase®, Flavourzyme®, Protamex®, and Papain under the optimal condition of each enzyme. An improvement in solubility was observed. Chicken trachea hydrolysates (CTH) with the highest solubility were detected in samples hydrolyzed with Alcalase®, follow by Protamex®, Papain, and Flavourzyme®, respectively. Within an hour of hydrolysis, the CTH obtained from Alcalase® hydrolysis had the highest total amino acid contents. The top three amino acids found in this hydrolyzate powder were Glutamic acid (Glu), Glycine (Gly), and Aspartic acid (Asp). Moreover, this sample exhibited the greatest antioxidant and bioactive properties as shown by the highest antioxidant capacity from DPPH and FRAP assay (4.42 TEAC µMol/mg CTH and 22.48 TEAC µMol/mg CTH, respectively) and the lowest IC50of ACE I inhibitor (0.41 mg/mL). These results suggested that Alcalase® hydrolysis provided the chicken trachea collagen hydrolyzate with unique properties that could be used in various food systems.
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作者：
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