Identification and characterization of miRNA transcriptome in potato by high-throughput sequencing.
Identification and characterization of miRNA transcriptome in potato by high-throughput sequencing.
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DOI：
10.1371/journal.pone.0057233
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Hornyik C
中科院分区：
文献类型：
--
作者：
Zhang R;Marshall D;Bryan GJ;Hornyik C
微小RNA（microRNAs，miRNAs）是一类短的、非编码的内源性RNA，在基因表达的转录后调控中发挥重要作用。虽然miRNAs在模式植物中的不同功能已经得到了很好的研究，但miRNAs在作物生物学中的影响却知之甚少。本研究采用高通量测序和生物信息学分析方法对马铃薯（Solanum tuberosum）中的miRNAs进行了分析。从叶和匍匐茎组织中分析小RNA。共发现28个保守的miRNA家族，并通过RNA凝胶印迹杂交鉴定了马铃薯特异性miRNA。描述了保守和马铃薯特异性miRNA的大小、来源和预测的靶点。马铃薯中大量的miRNAs和复杂的小分子RNA群体表明这些非编码RNA在多种生理和代谢途径中发挥重要作用。
Micro RNAs (miRNAs) represent a class of short, non-coding, endogenous RNAs which play important roles in post-transcriptional regulation of gene expression. While the diverse functions of miRNAs in model plants have been well studied, the impact of miRNAs in crop plant biology is poorly understood. Here we used high-throughput sequencing and bioinformatics analysis to analyze miRNAs in the tuber bearing crop potato (Solanum tuberosum). Small RNAs were analysed from leaf and stolon tissues. 28 conserved miRNA families were found and potato-specific miRNAs were identified and validated by RNA gel blot hybridization. The size, origin and predicted targets of conserved and potato specific miRNAs are described. The large number of miRNAs and complex population of small RNAs in potato suggest important roles for these non-coding RNAs in diverse physiological and metabolic pathways.
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影响因子：
14.9
作者：
Kozomara A;Griffiths-Jones S
通讯作者：
Griffiths-Jones S
影响因子：
14.9
作者：
Dai X;Zhao PX
通讯作者：
Zhao PX
影响因子：
3.7
作者：
Fahlgren N;Howell MD;Kasschau KD;Chapman EJ;Sullivan CM;Cumbie JS;Givan SA;Law TF;Grant SR;Dangl JL;Carrington JC
通讯作者：
Carrington JC
影响因子：
12.3
作者：
Langmead B;Trapnell C;Pop M;Salzberg SL
通讯作者：
Salzberg SL
DOI：
10.1073/pnas.1200169109
发表时间：
2012-02-14
影响因子：
11.1
作者：
Manavella, Pablo A.;Koenig, Daniel;Weigel, Detlef
通讯作者：
Weigel, Detlef