A Comparative Study of Embedded and Anesthetized Zebrafish in vivo on Myocardiac Calcium Oscillation and Heart Muscle Contraction.
A Comparative Study of Embedded and Anesthetized Zebrafish in vivo on Myocardiac Calcium Oscillation and Heart Muscle Contraction.
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DOI：
10.3389/fphar.2010.00139
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发表时间：
2010
影响因子：
5.6
通讯作者：
Nauli SM
中科院分区：
文献类型：
--
作者：
Muntean BS;Horvat CM;Behler JH;Aboualaiwi WA;Nauli AM;Williams FE;Nauli SM
斑马鱼（Danio rerio）已被用作研究脊椎动物心血管系统发育的模型。为了监测心脏收缩和胞质钙振荡，将鱼包埋在甲基纤维素中或用三卡因麻醉。使用高分辨率差分干涉对比和钙成像显微镜，我们在这里表明，多巴胺和维拉帕米改变钙信号和肌肉收缩麻醉斑马鱼，但不是嵌入式斑马鱼。在麻醉的鱼，多巴胺增加的幅度细胞溶质钙振荡随后增加心脏收缩，而维拉帕米降低钙振荡和心率的频率。有趣的是，维拉帕米也增加心肌收缩。我们的数据进一步表明，维拉帕米可以增加麻醉鱼心肌钙敏感性。总之，我们的数据加强了体内心脏对多巴胺和维拉帕米的反应。此外，多巴胺和维拉帕米对心肌钙和收缩的影响在麻醉比嵌入鱼更大。我们认为，虽然斑马鱼是一个很好的模型，心血管成像研究，心脏药理学的配置文件是非常不同的麻醉和嵌入鱼。
The zebrafish (Danio rerio) has been used as a model for studying vertebrate development in the cardiovascular system. In order to monitor heart contraction and cytosolic calcium oscillations, fish were either embedded in methylcellulose or anesthetized with tricaine. Using high-resolution differential interference contrast and calcium imaging microscopy, we here show that dopamine and verapamil alter calcium signaling and muscle contraction in anesthetized zebrafish, but not in embedded zebrafish. In anesthetized fish, dopamine increases the amplitude of cytosolic calcium oscillation with a subsequent increase in heart contraction, whereas verapamil decreases the frequency of calcium oscillation and heart rate. Interestingly, verapamil also increases myocardial contraction. Our data further indicate that verapamil can increase myocardial calcium sensitivity in anesthetized fish. Taken together, our data reinforce in vivo cardiac responses to dopamine and verapamil. Furthermore, effects of dopamine and verapamil on myocardial calcium and contraction are greater in anesthetized than embedded fish. We suggest that while the zebrafish is an excellent model for a cardiovascular imaging study, the cardio-pharmacological profiles are very different between anesthetized and embedded fish.
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影响因子：
4.5
作者：
Kolanczyk, RC;Fitzsimmons, PN;Schmieder, PK
通讯作者：
Schmieder, PK
影响因子：
7.3
作者：
MULVANY, MJ;NYBORG, N
通讯作者：
NYBORG, N
DOI：
10.1152/ajpheart.00870.2002
发表时间：
2003-04-01
影响因子：
4.8
作者：
Sedmera, D;Reckova, M;Thompson, RP
通讯作者：
Thompson, RP
影响因子：
14.8
作者：
Burns, CG;Milan, DJ;Fishman, MC
通讯作者：
Fishman, MC
脊椎动物心脏传导系统的遗传和生理解剖。
影响因子：
9.8
作者：
Chi NC;Shaw RM;Jungblut B;Huisken J;Ferrer T;Arnaout R;Scott I;Beis D;Xiao T;Baier H;Jan LY;Tristani-Firouzi M;Stainier DY
通讯作者：
Stainier DY