Diagnostic value of a plasma microRNA signature in gastric cancer: a microRNA expression analysis.
Diagnostic value of a plasma microRNA signature in gastric cancer: a microRNA expression analysis.
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胃癌血浆 microRNA 特征的诊断价值：microRNA 表达分析
DOI：
10.1038/srep11251
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发表时间：
2015-06-10
影响因子：
4.6
通讯作者：
Liu P
中科院分区：
文献类型：
--
作者：
Zhou X;Zhu W;Li H;Wen W;Cheng W;Wang F;Wu Y;Qi L;Fan Y;Chen Y;Ding Y;Xu J;Qian J;Huang Z;Wang T;Zhu D;Shu Y;Liu P
微小RNAs(MiRNAs)在胃癌患者血浆中的差异表达可作为一种诊断生物标志物。通过使用基于Exiqon Panel(miRCURY-Ready-to-Use-PCR-Human-panel-I + II-V1.M).的定量逆转录聚合酶链式反应(qRT-PCR)，通过初始筛选阶段(3个GC池与1个正常对照池)，共鉴定出33个miRNA通过qRT-PCR，这些miRNAs在训练中被进一步评估(30GC vs.30个NC)和测试阶段(71个GC与61 NCS)。我们发现了一个包括5个上调的miRNAs(miR-185、miR-20a、miR-210、miR-25和miR-92b)的血浆miRNA特征，该特征被评估为潜在的GC诊断标志物。对于训练、测试和外部验证阶段，签名的接收者操作特征曲线下的面积分别为0.86、0.74和0.87(32GC vs.18个NCS)。这5个 = 在胃癌组织中一直处于失调状态(N MiRNA30)。此外，动脉血中miR-185降低，而miR-20a、miR-210和miR-92b升高(n = 38)。然而，10例GC患者和10例NCS患者外显体中的miRNAs均无差异表达。总之，我们在外周血浆中发现了一个5-miRNA特征，可以作为检测GC的非侵入性生物标志物。
The differential expression of microRNAs (miRNAs) in plasma of gastric cancer (GC) patients may serve as a diagnostic biomarker. A total of 33 miRNAs were identified through the initial screening phase (3 GC pools vs. 1 normal control (NC) pool) using quantitative reverse transcription polymerase chain reaction (qRT-PCR) based Exiqon panel (miRCURY-Ready-to-Use-PCR-Human-panel-I + II-V1.M). By qRT-PCR, these miRNAs were further assessed in training (30 GC VS. 30 NCs) and testing stages (71 GC VS. 61 NCs). We discovered a plasma miRNA signature including five up-regulated miRNAs (miR-185,miR-20a,miR-210,miR-25andmiR-92b) and this signature was evaluated to be a potential diagnostic marker of GC. The areas under the receiver operating characteristic curve of the signature were 0.86, 0.74 and 0.87 for the training, testing and the external validation stages (32 GC VS. 18 NCs), respectively. The five miRNAs were consistently dysregulated in GC tissues (n = 30). Moreover,miR-185was decreased whilemiR-20a,miR-210andmiR-92bwere increased in arterial plasma (n = 38). However, none of the miRNAs in the exosomes showed different expression between 10 GC patients and 10 NCs. In conclusion, we identified a five-miRNA signature in the peripheral plasma which could serve as a non-invasive biomarker in detection of GC.
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影响因子：
8.8
作者：
Rotkrua P;Shimada S;Mogushi K;Akiyama Y;Tanaka H;Yuasa Y
通讯作者：
Yuasa Y
鉴定血清 microRNA 作为胃癌早期检测的新型非侵入性生物标志物。
影响因子：
3.7
作者：
Song MY;Pan KF;Su HJ;Zhang L;Ma JL;Li JY;Yuasa Y;Kang D;Kim YS;You WC
通讯作者：
You WC
影响因子：
5.6
作者：
Li X;Zhang Z;Yu M;Li L;Du G;Xiao W;Yang H
通讯作者：
Yang H
影响因子：
16.6
作者：
Hamilton MP;Rajapakshe K;Hartig SM;Reva B;McLellan MD;Kandoth C;Ding L;Zack TI;Gunaratne PH;Wheeler DA;Coarfa C;McGuire SE
通讯作者：
McGuire SE
影响因子：
3.7
作者：
Kirschner MB;Kao SC;Edelman JJ;Armstrong NJ;Vallely MP;van Zandwijk N;Reid G
通讯作者：
Reid G