An in-frame deletion mutation in the degron tail of auxin coreceptor IAA2 confers resistance to the herbicide 2,4-D in Sisymbrium orientale.
An in-frame deletion mutation in the degron tail of auxin coreceptor IAA2 confers resistance to the herbicide 2,4-D in Sisymbrium orientale.
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DOI：
10.1073/pnas.2105819119
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发表时间：
2022-03-01
影响因子：
11.1
通讯作者：
Gaines TA
中科院分区：
文献类型：
--
作者：
Figueiredo MRA;Küpper A;Malone JM;Petrovic T;Figueiredo ABTB;Campagnola G;Peersen OB;Prasad KVSK;Patterson EL;Reddy ASN;Kubeš MF;Napier R;Dayan FE;Preston C;Gaines TA
合成生长素除草剂交叉基础植物发育生物学和应用杂草管理。我们调查了2，4-D在澳大利亚杂草东方大蒜芥（印度绿篱芥菜）。我们确定了一个机制，涉及在生长素辅助受体IAA 2，Aux/IAA生长素辅助受体的基因家族的成员之一的降解决定子尾部的框内27-bp的缺失。我们表明，这种缺失IAA 2是一种功能获得性突变，赋予合成生长素抗性。这场进化的机制合成生长素除草剂的抗性证实了以前的生物化学研究显示的作用的Aux/IAA降解决定子尾巴在调节Aux/IAA蛋白降解生长素感知。使用基因编辑方法可以在作物中产生缺失突变，从而对多种合成生长素除草剂产生交叉抗性。天然生长素吲哚-3-乙酸（IAA）是植物生长发育的重要调节因子。合成生长素除草剂如2，4-D通过诱导植物中强烈的生长素信号应答来模拟IAA的作用。为了确定2，4-D抗性在Ssymophylla orientale（印度绿篱芥菜）杂草种群中的机制，我们对2，4-D抗性（R）和敏感（S）基因型进行了转录组分析，发现生长素辅助受体Aux/IAA 2（SoIAA 2）的降解决定子尾（DT）中有一个27个核苷酸的框内缺失，删除了9个氨基酸。在重组自交系中，缺失等位基因与2，4-D抗性共分离。此外，这种缺失也被检测到在几个2，4-D抗性田间种群的这个物种。表达SoIAA 2突变等位基因的拟南芥转基因株系对2，4-D和麦草畏具有抗性。IAA 2-DT缺失减少了与TIR 1在体外与天然和合成生长素的结合，导致缔合减少和解离速率增加。这种合成生长素除草剂抗性的机制将植物内功能分配给这种Aux/IAA辅助受体的DT区域，以用于其在合成生长素结合动力学中的作用，并揭示了使用基因编辑生产合成生长素抗性作物的潜在生物技术方法。
Synthetic auxin herbicides intersect basic plant developmental biology and applied weed management. We investigated resistance to 2,4-D in the Australian weed Sisymbrium orientale (Indian hedge mustard). We identified a mechanism involving an in-frame 27-bp deletion in the degron tail of auxin coreceptor IAA2, one member of the gene family of Aux/IAA auxin co-receptors. We show that this deletion in IAA2 is a gain-of-function mutation that confers synthetic auxin resistance. This field-evolved mechanism of resistance to synthetic auxin herbicides confirms previous biochemical studies showing the role of the Aux/IAA degron tail in regulating Aux/IAA protein degradation upon auxin perception. The deletion mutation could be generated in crops using gene-editing approaches for cross-resistance to multiple synthetic auxin herbicides. The natural auxin indole-3-acetic acid (IAA) is a key regulator of many aspects of plant growth and development. Synthetic auxin herbicides such as 2,4-D mimic the effects of IAA by inducing strong auxinic-signaling responses in plants. To determine the mechanism of 2,4-D resistance in a Sisymbrium orientale (Indian hedge mustard) weed population, we performed a transcriptome analysis of 2,4-D-resistant (R) and -susceptible (S) genotypes that revealed an in-frame 27-nucleotide deletion removing nine amino acids in the degron tail (DT) of the auxin coreceptor Aux/IAA2 (SoIAA2). The deletion allele cosegregated with 2,4-D resistance in recombinant inbred lines. Further, this deletion was also detected in several 2,4-D-resistant field populations of this species. Arabidopsis transgenic lines expressing the SoIAA2 mutant allele were resistant to 2,4-D and dicamba. The IAA2-DT deletion reduced binding to TIR1 in vitro with both natural and synthetic auxins, causing reduced association and increased dissociation rates. This mechanism of synthetic auxin herbicide resistance assigns an in planta function to the DT region of this Aux/IAA coreceptor for its role in synthetic auxin binding kinetics and reveals a potential biotechnological approach to produce synthetic auxin-resistant crops using gene-editing.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
56.9
作者：
Behrens, Mark R.;Mutlu, Nedim;Weeks, Donald P.
通讯作者：
Weeks, Donald P.
影响因子：
4.1
作者：
Hue Thi Dang;Malone, Jenna M.;Preston, Christopher
通讯作者：
Preston, Christopher
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
DOI：
10.1007/bf00331020
发表时间：
1986-01-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
HAUGHN, GW;SOMERVILLE, C
通讯作者：
SOMERVILLE, C