Stromal-derived factor-1 alpha-loaded PLGA microspheres for stem cell recruitment.
Stromal-derived factor-1 alpha-loaded PLGA microspheres for stem cell recruitment.
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DOI：
10.1007/s11095-011-0474-x
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发表时间：
2011-10
影响因子：
3.7
通讯作者：
Wang, Chun
中科院分区：
文献类型：
--
作者：
Cross, Daisy P.;Wang, Chun
基质衍生因子-1 α（SDF-1α）是一种化学引诱剂，已被研究用于治疗各种疾病，目的是将内源性干细胞招募到损伤部位。研究了可生物降解的PLGA微球作为以缓释方式递送SDF-1α的手段。我们采用双乳化溶剂萃取/蒸发技术将SDF-1α包埋在可生物降解的聚乳酸-乙醇酸共聚物（PLGA）微球中。通过改变微球的处方参数，考察了SDF-1α的体外释放特性、微球的大小和形态，并测定了SDF-1α的体外促骨髓间充质干细胞（MSCs）迁移活性。我们发现，使用封端的PLGA、BSA作为赋形剂和低溶剂体积制备的微球产生了高包封率（>64%），并在>50天的时间范围内释放SDF-1α。释放的SDF-1α具有生物活性，并在从微球释放的整个过程中引起MSC的显著迁移。我们已经确定了导致SDF-1α成功包封到PLGA微球中的几个变量。我们设想负载SDF-1 α的微球可以作为持续释放趋化因子的可注射来源，用于促进内源性干细胞向损伤部位的募集。
Stromal-derived factor-1 alpha (SDF-1α) is a chemo-attractant that has been investigated for treating various diseases, with the goal of recruiting endogenous stem cells to the site of injury. Biodegradable PLGA microspheres were investigated as a means to deliver SDF-1α in a sustained-release manner. We encapsulated SDF-1α into biodegradable poly (lactide-co-glycolide) (PLGA) microspheres using a double-emulsion solvent extraction/evaporation technique. We varied several formulation parameters, characterized the in vitro release profile of SDF-1α and the size and morphology of microspheres, and determined the bioactivity of the released SDF-1α of stimulating migration of mesenchymal stem cells (MSCs). We found that microspheres fabricated using end-capped PLGA, BSA as an excipient, and low solvent volumes yielded a high encapsulation efficiency (>64%) and released SDF-1α over a >50-day timeframe. The released SDF-1α was bioactive and caused significant migration of MSCs throughout the duration of release from the microspheres. We have identified several variables that led to successful encapsulation of SDF-1α into PLGA microspheres. We envision that SDF-lα-loaded microspheres may serve as injectable sources of sustained-release chemokine for promoting the recruitment of endogenous stem cells to the site of injury.
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巨核细胞对基质细胞衍生因子1（SDF-1）的跨内皮迁移增强了血小板的形成。
影响因子：
15.3
作者：
Hamada, T;Mohle, R;Hesselgesser, J;Hoxie, J;Nachman, R L;Moore, M A;Rafii, S
通讯作者：
Rafii, S
DOI：
10.1016/j.burns.2008.03.009
发表时间：
2009-03
期刊：
Burns : journal of the International Society for Burn Injuries
影响因子：
--
作者：
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通讯作者：
Jeschke MG
影响因子：
5.2
作者：
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通讯作者：
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影响因子：
37.8
作者：
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通讯作者：
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影响因子：
2.8
作者：
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通讯作者：
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