Alterations in osteoclast morphology following long-term 17beta-estradiol administration in the mouse.
Alterations in osteoclast morphology following long-term 17beta-estradiol administration in the mouse.
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DOI：
10.1186/1471-2121-2-3
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Gordon B
中科院分区：
文献类型：
--
作者：
Gruber HE;Puzanov IJ;Bennett M;Kumar V;Gordon B
尽管破骨细胞在骨吸收中的作用已被更好地理解，但关于破骨细胞生成和抗吸收剂（如17β-雌二醇）的确切作用机制仍有许多有待了解。本研究调查骨和破骨细胞形态学的改变后，长期雌激素管理的B6 D2 F1小鼠。4-5周龄的B6 D2 F1小鼠通过植入的硅橡胶管暴露于高水平的雌激素至少12周;对照组接受空管。对照组和给药组小鼠的股骨采用放射学、定量组织形态学和透射电子显微镜进行评估。治疗8周后，有严重骨质疏松的放射学证据，86%的股骨骨髓腔被骨替代。12周后，治疗动物的组织学研究表明，破骨细胞抗酒石酸酸性磷酸酶阳性，但显示出显着异常的超微结构，阻止成功的骨吸收。研究结果扩展了我们对小鼠体内暴露于高剂量雌激素的破骨细胞结构和功能的理解。超微结构检查显示，雌激素处理的小鼠破骨细胞不能密封骨表面，也不能形成皱褶的边界。
Although the role of the osteoclast in bone resorption is becoming better understood, much remains to be learned about osteoclastogenesis and the exact mechanism of action of anti-resorbing agents such as 17β-estradiol. This study investigated bone and morphologic osteoclast alterations following long-term estrogen administration to the B6D2F1 mouse. B6D2F1 mice aged 4-5 weeks were exposed to high levels of estrogen via implanted silastic tubing for at least 12 weeks; controls received empty tubing. Femurs of control and treated mice were assessed with radiology, quantitative histomorphometry and transmission electron microscopy. After 8 weeks of treatment, there was radiologic evidence of severe osteosclerosis and 86% of femoral marrow space was replaced with bone. After 12 weeks histologic studies of treated animals revealed that osteoclasts were positive for tartrate-resistant acid phosphatase but showed markedly abnormal ultrastructure which prevented successful bone resorption. Findings extend our understanding of osteoclast structure and function in the mouse exposed in vivo to high doses of estrogen. Ultrastructural examination showed that osteoclasts from estrogen-treated mice were unable to seal against the bone surface and were unable to form ruffled borders.
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DOI：
10.1159/000057436
发表时间：
1999-05-01
期刊：
MINERAL AND ELECTROLYTE METABOLISM
影响因子：
--
作者：
Gruber, HE;Norton, HJ;Singer, FR
通讯作者：
Singer, FR
DOI：
10.1007/bf00307190
发表时间：
1973-01-01
期刊：
ZEITSCHRIFT FUR ZELLFORSCHUNG UND MIKROSKOPISCHE ANATOMIE
影响因子：
--
作者：
LUCHT, U
通讯作者：
LUCHT, U
影响因子：
56.9
作者：
ERIKSEN, EF;COLVARD, DS;RIGGS, BL
通讯作者：
RIGGS, BL
影响因子：
56.9
作者：
JILKA, RL;HANGOC, G;MANOLAGAS, SC
通讯作者：
MANOLAGAS, SC
DOI：
10.1016/s0960-0760(96)00092-1
发表时间：
1996-10-01
影响因子：
4.1
作者：
Fiorelli, G;Gori, F;Brandi, ML
通讯作者：
Brandi, ML