Whole-Genome Sequencing of Staphylococcus aureus and Staphylococcus haemolyticus Clinical Isolates from Egypt.
Whole-Genome Sequencing of Staphylococcus aureus and Staphylococcus haemolyticus Clinical Isolates from Egypt.
复制标题
DOI：
10.1128/spectrum.02413-21
复制
发表时间：
2022-08-31
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
由耐药性葡萄球菌引起的感染是全球关注的问题。在中东也是如此，在那里检测到耐药性越来越强的金黄色葡萄球菌和溶血葡萄球菌菌株。虽然广泛的调查显示，在欧洲，亚洲和北美的耐药葡萄球菌引起的感染的患病率，耐药葡萄球菌的人口结构，从埃及的患者和临床环境中恢复仍然没有特征。我们对56个S. aureus和10株S.从亚历山大主大学医院分离的溶血性链球菌;金黄色葡萄球菌基因组和所有10个S.溶血性弧菌基因组携带mecA，其赋予甲氧西林抗性。补充了来自中东其他地区的额外的公开可用的基因组（34 S。aureus和6株S.溶血性葡萄球菌），我们提出了最大的基因组研究迄今为止的葡萄球菌菌株从中东。这些基因组包括20个S。金黄色葡萄球菌多位点序列型（MLST），包括3个新的MLST。其中包括9 S。溶血性MLST，包括1例新MLST。对每个物种核心基因组的系统基因组分析在很大程度上反映了MLST的系统基因组分析，而与地理来源无关。医院获得的spa t037/ST 239-SCCmec III/MLST CC 8克隆代表了最大的进化枝，占S.金黄色葡萄球菌分离株就像S。根据其他地区的金黄色葡萄球菌基因组调查，这些来自中东的分离株具有开放的泛基因组，这是毒力因子和抗生素抗性基因与其他宿主的基因交换的强有力指标。我们的基因组分析将为中东的抗生素管理和感染控制计划提供信息。重要性葡萄球菌尽管在中东流行，但研究不足。耐甲氧西林金黄色葡萄球菌（MRSA）是埃及医院的地方病，其他耐甲氧西林金黄色葡萄球菌菌株也是如此。金黄色葡萄球菌和溶血性为了深入了解在埃及流行的菌株，我们对56株S. aureus和10株S.亚历山大主大学医院的溶血性链球菌分离株。通过对这些基因组的分析，以及所有可用的S。金黄色葡萄球菌和从中东的溶血性弧菌基因组（n = 40），我们能够产生在这个地区的多样性的图片比传统的分子分型策略提供的更完整。例如，我们确定了4个新的MLST。大多数菌株携带与多药耐药、毒素产生、生物膜形成和免疫逃避相关的基因。这些数据为中东地区未来的抗生素管理和感染控制提供了宝贵的见解。
Infections caused by antibiotic-resistant Staphylococcus are a global concern. This is true in the Middle East, where increasingly resistant Staphylococcus aureus and Staphylococcus haemolyticus strains have been detected. While extensive surveys have revealed the prevalence of infections caused by antibiotic-resistant staphylococci in Europe, Asia, and North America, the population structure of antibiotic-resistant staphylococci recovered from patients and clinical settings in Egypt remains uncharacterized. We performed whole-genome sequencing of 56 S. aureus and 10 S. haemolyticus isolates from Alexandria Main University Hospital; 46 of the S. aureus genomes and all 10 of the S. haemolyticus genomes carry mecA, which confers methicillin resistance. Supplemented with additional publicly available genomes from the other parts of the Middle East (34 S. aureus and 6 S. haemolyticus), we present the largest genomic study to date of staphylococcal isolates from the Middle East. These genomes include 20 S. aureus multilocus sequence types (MLST), including 3 new ones. They also include 9 S. haemolyticus MLSTs, including 1 new one. Phylogenomic analyses of each species’ core genome largely mirrored those of the MLSTs, irrespective of geographical origin. The hospital-acquired spa t037/ST239-SCCmec III/MLST CC8 clone represented the largest clade, comprising 22% of the S. aureus isolates. Like S. aureus genome surveys of other regions, these isolates from the Middle East have an open pangenome, a strong indicator of gene exchange of virulence factors and antibiotic resistance genes with other reservoirs. Our genome analyses will inform antibiotic stewardship and infection control plans in the Middle East. IMPORTANCE Staphylococci are understudied despite their prevalence within the Middle East. Methicillin-resistant Staphylococcus aureus (MRSA) is endemic to hospitals in Egypt, as are other antibiotic-resistant strains of S. aureus and S. haemolyticus. To provide insight into the strains circulating in Egypt, we performed whole-genome sequencing of 56 S. aureus and 10 S. haemolyticus isolates from Alexandria Main University Hospital. Through analysis of these genomes, as well as all available S. aureus and S. haemolyticus genomes from the Middle East (n = 40), we were able to produce a picture of the diversity in this region more complete than those afforded by traditional molecular typing strategies. For example, we identified 4 new MLSTs. Most strains harbored genes associated with multidrug resistance, toxin production, biofilm formation, and immune evasion. These data provide invaluable insight for future antibiotic stewardship and infection control within the Middle East.
登录
查看更多内容
影响因子：
5.2
作者：
Asadollahi P;Farahani NN;Mirzaii M;Khoramrooz SS;van Belkum A;Asadollahi K;Dadashi M;Darban-Sarokhalil D
通讯作者：
Darban-Sarokhalil D
影响因子：
2.7
作者：
Delmont TO;Eren AM
通讯作者：
Eren AM
影响因子：
--
作者：
Abouelfetouh, Alaa
通讯作者：
Abouelfetouh, Alaa
影响因子：
5.2
作者：
Borg, Michael A.;de Kraker, Marlieke;Grundmann, Hajo
通讯作者：
Grundmann, Hajo
影响因子：
9.4
作者：
Bartels, Mette Damkjaer;Petersen, Andreas;Westh, Henrik
通讯作者：
Westh, Henrik