Bacteriocyte cell death in the pea aphid/Buchnera symbiotic system.
Bacteriocyte cell death in the pea aphid/Buchnera symbiotic system.
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DOI：
10.1073/pnas.1720237115
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发表时间：
2018-02-20
影响因子：
11.1
通讯作者：
Calevro F
中科院分区：
文献类型：
--
作者：
Simonet P;Gaget K;Balmand S;Ribeiro Lopes M;Parisot N;Buhler K;Duport G;Vulsteke V;Febvay G;Heddi A;Charles H;Callaerts P;Calevro F
在自然界中普遍存在的有益的共生关系导致了真核细胞的出现，细菌细胞，专门为微生物伴侣提供庇护。关于这些神秘细胞的最基本问题之一是生物体内平衡如何控制它们的消除。在这里，我们报告说，蚜虫细菌细胞已经演变成一种形式的细胞死亡，从保守的细胞死亡机制迄今为止的特点不同。这种细胞死亡机制是一个非凋亡的多步骤过程，始于内质网的超空泡化，随后是一系列细胞应激反应。我们的研究结果提供了一个框架，研究细菌细胞的生物功能和共生相关的细胞机制，并有助于了解真核细胞死亡的多样性。共生体在多细胞生命中起着关键作用，它促进了新性状的获得，扩大了生物体的生态能力。在强制性依赖于细胞内细菌共生体的昆虫中，新的宿主细胞（细菌细胞）或器官（细菌组）已经进化为窝藏有益的微生物伴侣。调节这些内共生体承载细胞的细胞生命周期的过程，例如控制它们的命运和消除以响应宿主生理的细胞死亡机制，是共生生物学中的基本问题。在这里，我们报告的半翅目昆虫Acyrthosiphon pisum的细菌细胞的退化过程中所涉及的细胞死亡过程的发现。这一过程在整个蚜虫成年期被逐步激活，并表现出与已知细胞死亡途径不同的形态学特征。通过结合电子显微镜，免疫组织化学和分子分析，我们证明了细菌细胞死亡的初始事件是非自噬空泡的细胞质积累，随后是一系列细胞应激反应，包括在空泡间隙形成自噬体，活性氧的激活，和Buchnera内共生体降解的溶酶体系统。我们发现，这个多步骤的细胞死亡过程起源于内质网，细胞器表现出独特的网状网络组织遍布整个细胞质和周围Buchnera aphidicola内共生体。我们的研究结果提供了深入的细胞和分子过程，协调真核宿主和内共生体的稳态和死亡的共生系统，并揭示了以前未知的方面的细菌细胞的生物功能。
Beneficial symbiotic associations, ubiquitously found in nature, have led to the emergence of eukaryotic cells, the bacteriocytes, specialized in harboring microbial partners. One of the most fundamental questions concerning these enigmatic cells is how organismal homeostasis controls their elimination. Here we report that aphid bacteriocytes have evolved a form of cell death distinct from the conserved cell-death mechanisms hitherto characterized. This cell-death mechanism is a nonapoptotic multistep process that starts with the hypervacuolation of the endoplasmic reticulum, followed by a cascade of cellular stress responses. Our findings provide a framework to study biological functioning of bacteriocytes and the cellular mechanisms associated with symbiosis and contribute to the understanding of eukaryotic cell-death diversity. Symbiotic associations play a pivotal role in multicellular life by facilitating acquisition of new traits and expanding the ecological capabilities of organisms. In insects that are obligatorily dependent on intracellular bacterial symbionts, novel host cells (bacteriocytes) or organs (bacteriomes) have evolved for harboring beneficial microbial partners. The processes regulating the cellular life cycle of these endosymbiont-bearing cells, such as the cell-death mechanisms controlling their fate and elimination in response to host physiology, are fundamental questions in the biology of symbiosis. Here we report the discovery of a cell-death process involved in the degeneration of bacteriocytes in the hemipteran insect Acyrthosiphon pisum. This process is activated progressively throughout aphid adulthood and exhibits morphological features distinct from known cell-death pathways. By combining electron microscopy, immunohistochemistry, and molecular analyses, we demonstrated that the initial event of bacteriocyte cell death is the cytoplasmic accumulation of nonautophagic vacuoles, followed by a sequence of cellular stress responses including the formation of autophagosomes in intervacuolar spaces, activation of reactive oxygen species, and Buchnera endosymbiont degradation by the lysosomal system. We showed that this multistep cell-death process originates from the endoplasmic reticulum, an organelle exhibiting a unique reticular network organization spread throughout the entire cytoplasm and surrounding Buchnera aphidicola endosymbionts. Our findings provide insights into the cellular and molecular processes that coordinate eukaryotic host and endosymbiont homeostasis and death in a symbiotic system and shed light on previously unknown aspects of bacteriocyte biological functioning.
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影响因子：
64.5
作者：
Dixon SJ;Lemberg KM;Lamprecht MR;Skouta R;Zaitsev EM;Gleason CE;Patel DN;Bauer AJ;Cantley AM;Yang WS;Morrison B 3rd;Stockwell BR
通讯作者：
Stockwell BR
影响因子：
11
作者：
Hosokawa, Takahiro;Nikoh, Naruo;Fukatsu, Takema
通讯作者：
Fukatsu, Takema
DOI：
10.1016/0020-1790(90)90064-2
发表时间：
1990-01-01
期刊：
INSECT BIOCHEMISTRY
影响因子：
--
作者：
HARA, E;FUKATSU, T;ISHIKAWA, H
通讯作者：
ISHIKAWA, H
DOI：
10.1073/pnas.062067299
发表时间：
2002-04-02
影响因子：
11.1
作者：
Gil, R;Sabater-Muñoz, B;Moya, A
通讯作者：
Moya, A
影响因子：
2.6
作者：
Legeai F;Shigenobu S;Gauthier JP;Colbourne J;Rispe C;Collin O;Richards S;Wilson AC;Murphy T;Tagu D
通讯作者：
Tagu D