Distal Bias of Meiotic Crossovers in Hexaploid Bread Wheat Reflects Spatio-Temporal Asymmetry of the Meiotic Program.
Distal Bias of Meiotic Crossovers in Hexaploid Bread Wheat Reflects Spatio-Temporal Asymmetry of the Meiotic Program.
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DOI：
10.3389/fpls.2021.631323
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发表时间：
2021
影响因子：
5.6
通讯作者：
Sanchez-Moran E
中科院分区：
文献类型：
--
作者：
Osman K;Algopishi U;Higgins JD;Henderson IR;Edwards KJ;Franklin FCH;Sanchez-Moran E
减数分裂重组产生遗传变异，并提供同源染色体之间的物理联系（交叉），这对于准确分离至关重要。在谷物中，交叉的分布（在细胞学上表现为交叉）偏向于染色体的远端区域。这给植物育种者开发具有改良农艺性状的品种造成了瓶颈，因为位于染色体间质和着丝粒近端区域的基因很少重组。小麦基因组学和基因组工程的最新进展与成熟的小麦细胞遗传学相结合，为操纵重组和解锁遗传变异提供了新的机会。作为这些研究的基础，我们利用染色体轴、联会复合体和重组蛋白的免疫定位，对六倍体小麦（Triticum aestivum）的减数分裂进展进行了详细分析。 5-Bromo-2'-脱氧尿苷 (BrdU) 标记用于确定与 DNA 复制相关的关键事件的时间顺序。轴形态发生、突触和重组起始被发现是时空协调的，从基因密集的远端染色体区域开始，随后发生在间质/近端区域。此外，远端区域的减数分裂进展与作为减数分裂特征的保守染色质周期相协调。这种交叉偏差的镜像在基因相关组蛋白标记 H3K4me3 和 H3K27me3 的分布中也很明显。重复相关标记，H3K27me1；和 H3K9me3。我们相信这项研究为功能研究和正在进行的操纵小麦基因组重组的举措提供了一个细胞遗传学框架。
Meiotic recombination generates genetic variation and provides physical links between homologous chromosomes (crossovers) essential for accurate segregation. In cereals the distribution of crossovers, cytologically evident as chiasmata, is biased toward the distal regions of chromosomes. This creates a bottleneck for plant breeders in the development of varieties with improved agronomic traits, as genes situated in the interstitial and centromere proximal regions of chromosomes rarely recombine. Recent advances in wheat genomics and genome engineering combined with well-developed wheat cytogenetics offer new opportunities to manipulate recombination and unlock genetic variation. As a basis for these investigations we have carried out a detailed analysis of meiotic progression in hexaploid wheat (Triticum aestivum) using immunolocalization of chromosome axis, synaptonemal complex and recombination proteins. 5-Bromo-2′-deoxyuridine (BrdU) labeling was used to determine the chronology of key events in relation to DNA replication. Axis morphogenesis, synapsis and recombination initiation were found to be spatio-temporally coordinated, beginning in the gene-dense distal chromosomal regions and later occurring in the interstitial/proximal regions. Moreover, meiotic progression in the distal regions was coordinated with the conserved chromatin cycles that are a feature of meiosis. This mirroring of the chiasma bias was also evident in the distribution of the gene-associated histone marks, H3K4me3 and H3K27me3; the repeat-associated mark, H3K27me1; and H3K9me3. We believe that this study provides a cytogenetic framework for functional studies and ongoing initiatives to manipulate recombination in the wheat genome.
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影响因子：
30.8
作者：
Choi, Kyuha;Zhao, Xiaohui;Kelly, Krystyna A.;Venn, Oliver;Higgins, James D.;Yelina, Nataliya E.;Hardcastle, Thomas J.;Ziolkowski, Piotr A.;Copenhaver, Gregory P.;Franklin, F. Chris H.;McVean, Gil;Henderson, Ian R.
通讯作者：
Henderson, Ian R.
影响因子：
4.5
作者：
Adam C;Guérois R;Citarella A;Verardi L;Adolphe F;Béneut C;Sommermeyer V;Ramus C;Govin J;Couté Y;Borde V
通讯作者：
Borde V
DOI：
10.1126/science.aaf4778
发表时间：
2017-01-27
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Ahuja JS;Sandhu R;Mainpal R;Lawson C;Henley H;Hunt PA;Yanowitz JL;Börner GV
通讯作者：
Börner GV
影响因子：
56.9
作者：
CHIKASHIGE, Y;DING, DQ;HIRAOKA, Y
通讯作者：
HIRAOKA, Y
影响因子：
56.9
作者：
Choulet, Frederic;Alberti, Adriana;Feuillet, Catherine
通讯作者：
Feuillet, Catherine