Transcript profiling of crown rootless1 mutant stem base reveals new elements associated with crown root development in rice.
Transcript profiling of crown rootless1 mutant stem base reveals new elements associated with crown root development in rice.
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DOI：
10.1186/1471-2164-12-387
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发表时间：
2011-08-01
期刊：
影响因子：
4.4
通讯作者：
Gantet P
中科院分区：
文献类型：
--
作者：
Coudert Y;Bès M;Le TV;Pré M;Guiderdoni E;Gantet P
水稻胚后根系统的主要部分是茎源性根，称为冠根（CR）。在水稻根发育受影响的少数特征突变体中，冠状无根突变体1不能启动冠状根原基。CRL 1是由生长素诱导的，编码一个AS 2/LOB结构域转录因子，作用于控制CR发育的基因调控网络的上游。为了确定参与CR发展的基因，我们比较了crl 1突变体和野生型（WT）植物茎基部的全球基因表达谱。我们的分析显示，250和236个基因分别下调和上调crl 1突变体。生长素诱导CRL 1表达，因此预期生长素也改变由CRL 1早期调节的基因的表达。为了鉴定CRL 1早期控制下的基因，我们监测了所选基因子集的表达动力学，主要选自那些表现出差异表达的基因，在外源生长素处理后的CRL 1和WT中。这些基因主要与激素、水分和营养、发育和稳态有关，可能不受CRL 1的直接调控。我们假设，这些基因的差异表达观察crl 1突变体可能是CR形成的情况下的后果。另外，还鉴定了3个CRL 1依赖的生长素应答基因：FSM（扁平茎分生组织）/FAS 1（FASCIATA 1）、GTE 4（一般转录因子组E4）和MAP（微管相关蛋白）。FSM/FAS 1和GTE 4在水稻和拟南芥中分别通过染色质重塑和细胞周期调控参与根分生组织的维持。我们的数据表明，大多数基因在crl 1与野生型的差异调节可能是一个间接的后果，CRL 1失活导致的CR在crl 1突变体的情况下。然而，一些基因，FAS 1/FSM，GTE 4和MAP，需要CRL 1被生长素诱导，这表明它们可能直接受CRL 1调节。这些基因具有与极化细胞生长、细胞周期调节或染色质重塑相关的功能。这表明这些基因受CRL 1控制，并参与水稻CR的启动。
In rice, the major part of the post-embryonic root system is made of stem-derived roots named crown roots (CR). Among the few characterized rice mutants affected in root development, crown rootless1 mutant is unable to initiate crown root primordia. CROWN ROOTLESS1 (CRL1) is induced by auxin and encodes an AS2/LOB-domain transcription factor that acts upstream of the gene regulatory network controlling CR development. To identify genes involved in CR development, we compared global gene expression profile in stem bases of crl1 mutant and wild-type (WT) plants. Our analysis revealed that 250 and 236 genes are down- and up-regulated respectively in the crl1 mutant. Auxin induces CRL1 expression and consequently it is expected that auxin also alters the expression of genes that are early regulated by CRL1. To identify genes under the early control of CRL1, we monitored the expression kinetics of a selected subset of genes, mainly chosen among those exhibiting differential expression, in crl1 and WT following exogenous auxin treatment. This analysis revealed that most of these genes, mainly related to hormone, water and nutrient, development and homeostasis, were likely not regulated directly by CRL1. We hypothesized that the differential expression for these genes observed in the crl1 mutant is likely a consequence of the absence of CR formation. Otherwise, three CRL1-dependent auxin-responsive genes: FSM (FLATENNED SHOOT MERISTEM)/FAS1 (FASCIATA1), GTE4 (GENERAL TRANSCRIPTION FACTOR GROUP E4) and MAP (MICROTUBULE-ASSOCIATED PROTEIN) were identified. FSM/FAS1 and GTE4 are known in rice and Arabidopsis to be involved in the maintenance of root meristem through chromatin remodelling and cell cycle regulation respectively. Our data showed that the differential regulation of most genes in crl1 versus WT may be an indirect consequence of CRL1 inactivation resulting from the absence of CR in the crl1 mutant. Nevertheless some genes, FAS1/FSM, GTE4 and MAP, require CRL1 to be induced by auxin suggesting that they are likely directly regulated by CRL1. These genes have a function related to polarized cell growth, cell cycle regulation or chromatin remodelling. This suggests that these genes are controlled by CRL1 and involved in CR initiation in rice.
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影响因子：
64.8
作者：
Matsumoto, T;Wu, JZ;Sugiura, M
通讯作者：
Sugiura, M
影响因子：
2.7
作者：
Abe, Masashi;Kuroshita, Hideaki;Nagato, Yasuo
通讯作者：
Nagato, Yasuo
影响因子：
5.1
作者：
Caldana C;Scheible WR;Mueller-Roeber B;Ruzicic S
通讯作者：
Ruzicic S
影响因子：
4.6
作者：
Exner, Vivien;Taranto, Patti;Hennig, Lars
通讯作者：
Hennig, Lars
DOI：
10.1073/pnas.1734110100
发表时间：
2003-09-02
影响因子：
11.1
作者：
Golovkin, M;Reddy, ASN
通讯作者：
Reddy, ASN