Fluorescently Labelled Silica Coated Gold Nanoparticles as Fiducial Markers for Correlative Light and Electron Microscopy.
Fluorescently Labelled Silica Coated Gold Nanoparticles as Fiducial Markers for Correlative Light and Electron Microscopy.
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DOI：
10.1038/s41598-018-31836-1
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发表时间：
2018-09-11
影响因子：
4.6
通讯作者：
Gerritsen HC
中科院分区：
文献类型：
--
作者：
Fokkema J;Fermie J;Liv N;van den Heuvel DJ;Konings TOM;Blab GA;Meijerink A;Klumperman J;Gerritsen HC
在这项工作中，金纳米粒子涂有荧光标记（罗丹明B）的二氧化硅外壳作为基准标记的相关光学和电子显微镜（CLEM）。对颗粒的合成进行优化，以获得任意尺寸的均匀球形核壳颗粒。接下来，用不同荧光团密度标记的颗粒被表征以确定在何种条件下可以获得明亮且（光）稳定的颗粒。2和3D CLEM的例子，其中优化的粒子用于相关性。在2D示例中，将基准添加到细胞的冷冻切片，而在3D示例中，在基准的内吞作用之后对细胞成像。这两个例子表明，颗粒在两种模式下都清晰可见，可以用于相关性。此外，基准点的可识别核壳结构在电子显微镜中被证明是非常强大的：它可以无可辩驳地识别颗粒，并可以轻松准确地确定基准点的中心。
In this work, gold nanoparticles coated with a fluorescently labelled (rhodamine B) silica shell are presented as fiducial markers for correlative light and electron microscopy (CLEM). The synthesis of the particles is optimized to obtain homogeneous, spherical core-shell particles of arbitrary size. Next, particles labelled with different fluorophore densities are characterized to determine under which conditions bright and (photo)stable particles can be obtained. 2 and 3D CLEM examples are presented where optimized particles are used for correlation. In the 2D example, fiducials are added to a cryosection of cells whereas in the 3D example cells are imaged after endocytosis of the fiducials. Both examples demonstrate that the particles are clearly visible in both modalities and can be used for correlation. Additionally, the recognizable core-shell structure of the fiducials proves to be very powerful in electron microscopy: it makes it possible to irrefutably identify the particles and makes it easy to accurately determine the center of the fiducials.
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影响因子：
2.2
作者：
Schellenberger, Pascale;Kaufmann, Rainer;Siebert, C. Alistair;Hagen, Christoph;Wodrich, Harald;Gruenewald, Kay
通讯作者：
Gruenewald, Kay
影响因子：
2.8
作者：
ARBELOA, IL;OJEDA, PR
通讯作者：
OJEDA, PR
影响因子：
4.1
作者：
Jin, Yuhui;Kannan, Shibichakravarthy;Zhao, Julia Xiaojun
通讯作者：
Zhao, Julia Xiaojun
影响因子：
2.3
作者：
Genovese, Damiano;Rampazzo, Enrico;Prodi, Luca
通讯作者：
Prodi, Luca
影响因子：
3.9
作者：
Graf, C;Vossen, DLJ;van Blaaderen, A
通讯作者：
van Blaaderen, A