Identification of a xylose reductase gene in the xylose metabolic pathway of Kluyveromyces marxianus NBRC1777
Identification of a xylose reductase gene in the xylose metabolic pathway of Kluyveromyces marxianus NBRC1777
复制标题
马克斯克鲁维酵母NBRC1777木糖代谢途径中木糖还原酶基因的鉴定
DOI：
10.1007/s10295-011-0990-z
复制
发表时间：
2011-06
影响因子：
3.4
通讯作者：
Hong, Jiong
中科院分区：
文献类型：
--
作者：
Zhang, Biao;Zhang, Ling;Wang, Dongmei;Gao, Xiaolian;Hong, Jiong
马克斯克鲁维酵母是一种耐热酵母，能够利用更广泛的底物，并且比大多数其他酵母物种具有更大的耐热性。K. Marxianus能同化木糖，但在氧气有限的环境中由木糖生产乙醇的能力很差。在本研究中，K.为阐明限制马克斯酵母木糖发酵的因素，克隆了马克斯酵母木糖还原酶（KmXR）基因（Kmxyl 1）。marxianus NBRC1777. KmXR是K. marxianus，这是通过破坏Kmxyl 1基因验证的。重组KmXR对NADPH的Km为65.67 μM，KmXR活性为1.295 U/mg，低于已报道的大多数酵母XR，且对辅酶NADH无活性。这一结果表明，来自K. marxianus是高度辅酶特异性的，结合K. marxianus与NADP+，木糖发酵的限制是由于在厌氧条件下的氧化还原不平衡和低KmXR活性。
Kluyveromyces marxianus is thermotolerant yeast that is able to utilize a wider range of substrates and has greater thermal tolerance than most other yeast species. K. marxianus can assimilate xylose, but its ability to produce ethanol from xylose in oxygen-limited environments is poor. In the present study, the K. marxianus xylose reductase (KmXR) gene (Kmxyl1) was cloned and the recombinant enzyme was characterized to clarify the factors that limit xylose fermentation in K. marxianus NBRC1777. KmXR is a key enzyme in the xylose metabolism of K. marxianus, which was verified by disruption of the Kmxyl1 gene. The Km of the recombinant KmXR for NADPH is 65.67 μM and KmXR activity is 1.295 U/mg, which is lower than those of most reported yeast XRs, and the enzyme has no activity with coenzyme NADH. This result demonstrates that the XR from K. marxianus is highly coenzyme specific; combined with the extremely low XDH activity of K. marxianus with NADP+, the limitation of xylose fermentation is due to a redox imbalance under anaerobic conditions and low KmXR activity.
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DOI：
10.1271/bbb.80853
发表时间：
2009-05
期刊：
Bioscience, Biotechnology, and Biochemistry
影响因子：
--
作者：
Sanom Nonklang;A. Ano;B. Abdel-Banat;Yuko Saito;H. Hoshida;R. Akada
通讯作者：
Sanom Nonklang;A. Ano;B. Abdel-Banat;Yuko Saito;H. Hoshida;R. Akada
影响因子：
3.8
作者：
F. C. Sampaio;J. T. Faria;J. Coimbra;Flávia M. Lopes Passos;A. Converti;L. A. Minin
通讯作者：
F. C. Sampaio;J. T. Faria;J. Coimbra;Flávia M. Lopes Passos;A. Converti;L. A. Minin
影响因子：
3.4
作者：
F. C. Sampaio;J. T. Faria;Flávia M. Lopes Passos;A. Converti;L. A. Minin
通讯作者：
F. C. Sampaio;J. T. Faria;Flávia M. Lopes Passos;A. Converti;L. A. Minin
影响因子：
4.1
作者：
VERDUYN, C;VANKLEEF, R;SCHEFFERS, WA
通讯作者：
SCHEFFERS, WA
影响因子：
2.7
作者：
Xiaoxia Wang;B. Fang;Juxiang Luo;Wenjun Li;Liaoyuan Zhang
通讯作者：
Xiaoxia Wang;B. Fang;Juxiang Luo;Wenjun Li;Liaoyuan Zhang