Antiadhesive role of apical decay-accelerating factor (CD55) in human neutrophil transmigration across mucosal epithelia.
Antiadhesive role of apical decay-accelerating factor (CD55) in human neutrophil transmigration across mucosal epithelia.
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DOI：
10.1084/jem.20030380
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发表时间：
2003-10-06
期刊：
影响因子：
--
通讯作者：
Colgan SP
中科院分区：
文献类型：
--
作者：
Lawrence DW;Bruyninckx WJ;Louis NA;Lublin DM;Stahl GL;Parkos CA;Colgan SP
炎症发作期间中性粒细胞跨粘膜上皮的迁移涉及许多细胞表面分子和信号通路的精确协调。成功迁移到顶端上皮表面后，顶端定位的上皮蛋白可能有助于将中性粒细胞保留在管腔表面。目前，顶端上皮配体及其 PMN 反受体的鉴定仍然难以捉摸。因此，为了确定参与中性粒细胞-上皮相互作用的顶端上皮细胞表面蛋白的存在，我们筛选了一组针对上皮质膜的抗体。该策略鉴定出一种抗体 (OE-1)，该抗体既定位于顶端细胞膜，又显着抑制 PMN 跨上皮单层的迁移。微序列分析表明，OE-1 识别人类腐烂加速因子（DAF、CD55）。 DAF 是一种高度糖基化的 70-80 kD、糖基磷脂酰肌醇连接蛋白，主要作为自体补体裂解抑制剂发挥作用。使用反义寡核苷酸或 RNA 干扰的 DAF 抑制实验表明，DAF 可能充当抗粘附分子，促进 PMN 迁移后从管腔表面释放。类似地，对应于 OE-1 抗原识别结构域的肽导致 PMN 在顶端上皮表面积累。 DAF 作为 PMN 顶端上皮配体的阐明为旨在平息不需要的炎症发作的新型抗炎疗法提供了靶标。
Neutrophil migration across mucosal epithelium during inflammatory episodes involves the precise orchestration of a number a cell surface molecules and signaling pathways. After successful migration to the apical epithelial surface, apically localized epithelial proteins may serve to retain PMN at the lumenal surface. At present, identification of apical epithelial ligands and their PMN counter-receptors remain elusive. Therefore, to define the existence of apical epithelial cell surface proteins involved in PMN–epithelial interactions, we screened a panel of antibodies directed against epithelial plasma membranes. This strategy identified one antibody (OE-1) that both localized to the apical cell membrane and significantly inhibited PMN transmigration across epithelial monolayers. Microsequence analysis revealed that OE-1 recognized human decay-accelerating factor (DAF, CD55). DAF is a highly glycosylated, 70–80-kD, glycosyl-phosphatidyinositol–linked protein that functions predominantly as an inhibitor of autologous complement lysis. DAF suppression experiments using antisense oligonucleotides or RNA interference revealed that DAF may function as an antiadhesive molecule promoting the release of PMN from the lumenal surface after transmigration. Similarly, peptides corresponding to the antigen recognition domain of OE-1 resulted in accumulation of PMN on the apical epithelial surface. The elucidation of DAF as an apical epithelial ligand for PMN provides a target for novel anti-inflammatory therapies directed at quelling unwanted inflammatory episodes.
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影响因子：
4.4
作者：
Kucik, DF
通讯作者：
Kucik, DF
影响因子：
4.8
作者：
Collard, CD;Park, KA;Colgan, SP
通讯作者：
Colgan, SP
影响因子：
5.4
作者：
KING, PD;BATCHELOR, AH;KATZ, DR
通讯作者：
KATZ, DR
DOI：
10.1007/bf01872889
发表时间：
1990-03
期刊：
The Journal of membrane biology
影响因子：
--
作者：
Lisanti MP;Le Bivic A;Saltiel AR;Rodriguez-Boulan E
通讯作者：
Rodriguez-Boulan E
影响因子：
--
作者：
COLGAN, SP;PARKOS, CA;MADARA, JL
通讯作者：
MADARA, JL