Mechanical robustness of Pseudomonas aeruginosa biofilms.

Mechanical robustness of Pseudomonas aeruginosa biofilms.
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DOI:
10.1039/c0sm01467b
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发表时间:
2011
期刊:
影响因子:
3.4
通讯作者:
Ribbeck K
Ribbeck K
中科院分区:
化学2区
文献类型:
--
作者:
Lieleg O;Caldara M;Baumgärtel R;Ribbeck K

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生物膜在各种表面和许多不同环境中生长,这种现象构成了工业和医学中的主要问题。尽管生物膜很重要,但人们对生物膜的粘弹性特性以及它们如何依赖于化学微环境却知之甚少。在这里,我们发现铜绿假单胞菌(P.a.)生物膜的机械特性对于化学扰动具有高度鲁棒性。具体来说,我们观察到 P.a.即使在化学品存在的情况下,生物膜在屈服后也能够完全恢复其初始硬度。此外,只有三价离子和柠檬酸显着影响生物膜弹性,其中第一个也会改变材料的质地。最后,我们的结果表明生物膜力学和生物膜内的细菌活力没有必然联系,这表明单独针对细菌可能不足以用于生物膜去除策略。
Biofilms grow on various surfaces and in many different environments, a phenomenon that constitutes major problems in industry and medicine. Despite their importance little is known about the viscoelastic properties of biofilms and how these depend on the chemical microenvironment. Here, we find that the mechanical properties of Pseudomonas aeruginosa (P.a.) biofilms are highly robust towards chemical perturbations. Specifically, we observe that P.a. biofilms are able to fully regain their initial stiffness after yielding is enforced, even in the presence of chemicals. Moreover, only trivalent ions and citric acid significantly affect the biofilm elasticity, the first of which also alter the texture of the material. Finally, our results indicate that biofilm mechanics and bacteria viability inside the biofilm are not necessarily linked which suggests that targeting bacteria alone might not be sufficient for biofilm removal strategies.
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