In vitro susceptibility of established biofilms composed of a clinical wound isolate of Pseudomonas aeruginosa treated with lactoferrin and xylitol.
In vitro susceptibility of established biofilms composed of a clinical wound isolate of Pseudomonas aeruginosa treated with lactoferrin and xylitol.
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DOI：
10.1016/j.ijantimicag.2008.08.013
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发表时间：
2009-03
影响因子：
10.8
通讯作者：
James GA
中科院分区：
文献类型：
--
作者：
Ammons MC;Ward LS;Fisher ST;Wolcott RD;James GA
细菌生物膜的医学影响随着生物膜作为慢性伤口（例如糖尿病足溃疡、静脉性腿部溃疡和压力性溃疡）的主要贡献者的认识而增加。传统的治疗方法已被证明是无效的，因此本文提出了体外证据，以支持使用新型抗菌药物治疗铜绿假单胞菌生物膜。对具有铜绿假单胞菌临床分离株的体外生物膜模型进行单独或组合的乳铁蛋白或木糖醇处理。组合的乳铁蛋白和木糖醇处理破坏了铜绿假单胞菌生物膜的结构，并导致活力降低>2log。原位分析表明，虽然木糖醇处理似乎破坏生物膜结构，但乳铁蛋白处理导致透化细菌细胞数量增加两倍以上。本文提出的发现表明，用乳铁蛋白和木糖醇的组合处理显著降低了体外建立的铜绿假单胞菌生物膜的活力，并且该处理的抗微生物机制包括生物膜结构破坏和细菌膜的透化。
The medical impact of bacterial biofilms has increased with the recognition of biofilms as a major contributor to chronic wounds such as diabetic foot ulcers, venous leg ulcers and pressure ulcers. Traditional methods of treatment have proven ineffective, therefore this article presents in vitro evidence to support the use of novel antimicrobials in the treatment of Pseudomonas aeruginosa biofilm. An in vitro biofilm model with a clinical isolate of P. aeruginosa was subjected to treatment with either lactoferrin or xylitol alone or in combination. Combined lactoferrin and xylitol treatment disrupted the structure of the P. aeruginosa biofilm and resulted in a >2log reduction in viability. In situ analysis indicated that while xylitol treatment appeared to disrupt the biofilm structure, lactoferrin treatment resulted in a greater than two-fold increase in the number of permeabilised bacterial cells. The findings presented here indicated that combined treatment with lactoferrin and xylitol significantly decreases the viability of established P. aeruginosa biofilms in vitro and that the antimicrobial mechanism of this treatment includes both biofilm structural disruption and permeablisation of bacterial membranes.
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影响因子：
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