Basic domains target protein subunits of the RNase MRP complex to the nucleolus independently of complex association.
Basic domains target protein subunits of the RNase MRP complex to the nucleolus independently of complex association.
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基本结构域将 RNase MRP 复合物的蛋白质亚基靶向核仁，与复合物的关联无关。
DOI：
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发表时间：
2001
影响因子：
3.3
通讯作者：
G. Pruijn
中科院分区：
文献类型：
--
作者：
H. Eenennaam;A. Heijden;R. Janssen;W. Venrooij;G. Pruijn
The RNase MRP and RNase P ribonucleoprotein particles both function as endoribonucleases, have a similar RNA component, and share several protein subunits. RNase MRP has been implicated in pre-rRNA processing and mitochondrial DNA replication, whereas RNase P functions in pre-tRNA processing. Both RNase MRP and RNase P accumulate in the nucleolus of eukaryotic cells. In this report we show that for three protein subunits of the RNase MRP complex (hPop1, hPop4, and Rpp38) basic domains are responsible for their nucleolar accumulation and that they are able to accumulate in the nucleolus independently of their association with the RNase MRP and RNase P complexes. We also show that certain mutants of hPop4 accumulate in the Cajal bodies, suggesting that hPop4 traverses through these bodies to the nucleolus. Furthermore, we characterized a deletion mutant of Rpp38 that preferentially associates with the RNase MRP complex, giving a first clue about the difference in protein composition of the human RNase MRP and RNase P complexes. On the basis of all available data on nucleolar localization sequences, we hypothesize that nucleolar accumulation of proteins containing basic domains proceeds by diffusion and retention rather than by an active transport process. The existence of nucleolar localization sequences is discussed.
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影响因子：
10.5
作者：
Chamberlain, JR;Lee, Y;Engelke, DR
通讯作者：
Engelke, DR
影响因子：
10.5
作者：
Bertrand, E;Houser-Scott, F;Engelke, DR
通讯作者：
Engelke, DR
RNase P 的 RNA 亚基的核结构域。
影响因子：
4
作者：
Jacobson,MR;Cao,LG;Taneja,K;Singer,RH;Wang,YL;Pederson,T
通讯作者：
Pederson,T
DOI：
10.1073/pnas.94.4.1101
发表时间：
1997-02-18
影响因子：
11.1
作者：
Eder, PS;Kekuda, R;Altman, S
通讯作者：
Altman, S
影响因子：
11.4
作者：
CHANG, DD;CLAYTON, DA
通讯作者：
CLAYTON, DA