In vivo optogenetic tracing of functional corticocortical connections between motor forelimb areas.
In vivo optogenetic tracing of functional corticocortical connections between motor forelimb areas.
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DOI：
10.3389/fncir.2013.00055
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发表时间：
2013
影响因子：
3.5
通讯作者：
Matsuzaki M
中科院分区：
文献类型：
--
作者：
Hira R;Ohkubo F;Tanaka YR;Masamizu Y;Augustine GJ;Kasai H;Matsuzaki M
不同运动皮层区域之间的相互作用对于协调运动行为是必不可少的。在啮齿类动物中，运动皮层前肢区被分为至少两个不同的区域：喙侧前肢区（RFA）和尾侧前肢区（CFA）。RFA被认为是灵长类动物运动前皮质（PM）的等价物，而CFA被认为是初级运动皮质的等价物。虽然在大鼠中已经在解剖学上确定了RFA和CFA之间的相互连接，但尚不清楚这些区域之间是否存在可以诱导突触后尖峰的功能连接。在这项研究中，我们使用了在体内的视紫红质-2（ChR 2）的光刺激方法来跟踪小鼠RFA和CFA之间的功能连接。同步电记录被用来检测尖峰活动引起的突触输入来自光刺激的地区。该方法结合解剖学追踪，证明RFA接收来自CFA的第2/3层和/或第5a层的强功能投射，但不接收来自CFA的第5 b层（L5 b）的强功能投射。此外，CFA接收来自RFA的L5 b神经元的强投射。在其他区域的光刺激后，在远程区域诱发的电反应的起始潜伏期约为10 ms，这与这些区域之间的突触连接性一致。我们的研究结果表明，在RFA和CFA运动过程中的神经元活动是通过不对称的相互连接。
Interactions between distinct motor cortical areas are essential for coordinated motor behaviors. In rodents, the motor cortical forelimb areas are divided into at least two distinct areas: the rostral forelimb area (RFA) and the caudal forelimb area (CFA). The RFA is thought to be an equivalent of the premotor cortex (PM) in primates, whereas the CFA is believed to be an equivalent of the primary motor cortex. Although reciprocal connections between the RFA and the CFA have been anatomically identified in rats, it is unknown whether there are functional connections between these areas that can induce postsynaptic spikes. In this study, we used an in vivo Channelrhodopsin-2 (ChR2) photostimulation method to trace the functional connections between the mouse RFA and CFA. Simultaneous electrical recordings were utilized to detect spiking activities induced by synaptic inputs originating from photostimulated areas. This method, in combination with anatomical tracing, demonstrated that the RFA receives strong functional projections from layer 2/3 and/or layer 5a, but not from layer 5b (L5b), of the CFA. Further, the CFA receives strong projections from L5b neurons of the RFA. The onset latency of electrical responses evoked in remote areas upon photostimulation of the other areas was approximately 10 ms, which is consistent with the synaptic connectivity between these areas. Our results suggest that neuronal activities in the RFA and the CFA during movements are formed through asymmetric reciprocal connections.
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