The multifaceted effects of polysaccharides isolated from Dendrobium huoshanense on immune functions with the induction of interleukin-1 receptor antagonist (IL-1ra) in monocytes.
The multifaceted effects of polysaccharides isolated from Dendrobium huoshanense on immune functions with the induction of interleukin-1 receptor antagonist (IL-1ra) in monocytes.
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DOI：
10.1371/journal.pone.0094040
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Wong CH
中科院分区：
文献类型：
--
作者：
Lin J;Chang YJ;Yang WB;Yu AL;Wong CH
霍山石斛是一种名贵、用途广泛的中草药，具有抗癌、抗炎等功效。然而，其免疫学活性仅限于对少数细胞因子和免疫细胞功能的体外研究。首先，我们研究了从水解液中提取的多糖(DHPS)在体内和体外对小鼠和人诱导一系列细胞因子/趋化因子的作用。我们发现，水解酶多糖在体内和体外均能诱导小鼠和人细胞产生TH1、TH2、炎性细胞因子和趋化因子。其次，在体内实验中，我们证明了水杨酸多糖可扩增小鼠脾细胞，包括CD4+T细胞、CD8+T细胞、B细胞、NK细胞、NKT细胞、单核/巨噬细胞、粒细胞和调节性T细胞。值得注意的是，DH-PS在小鼠和人类免疫细胞，特别是单核细胞中诱导了一种抗炎分子IL-1ra。注射DH-PS诱导的血清IL-1ra水平是IL-1β的10倍以上，提示DH-PS诱导的抗炎作用可能超过IL-1β介导的炎症作用。研究结果表明，DH-PS诱导IL-1ra产生的信号通路涉及ERK/ELK、p38MAPK、PI3K和NFκB。最后，我们观察到DH-PS诱导的IL-1ra水平显著高于另一种常用中草药灵芝多糖提取物F3。这些结果表明，水杨酸多糖可能在改善IL-1诱导的致病条件方面具有潜在的应用价值。
Dendrobium huoshanense is a valuable and versatile Chinese herbal medicine with the anecdotal claims of cancer prevention and anti-inflammation. However, its immunological activities are limited to in vitro studies on a few cytokines and immune cell functions. First, we investigated the effects of polysaccharides isolated from DH (DH-PS) on inducing a panel of cytokines/chemokines in mice in vivo and human in vitro. We found that DH polysaccharides (DH-PS) induced TH1, TH2, inflammatory cytokines and chemokines in mouse in vivo and human cells in vitro. Secondly, we demonstrated that DH-PS expanded mouse splenocytes in vivo including CD4+ T cells, CD8+ T cells, B cells, NK cells, NKT cells, monocytes/macrophages, granulocytes and regulatory T cells. Notably, DH-PS induced an anti-inflammatory molecule, IL-1ra, in mouse and human immune cells, especially monocytes. The serum level of IL-1ra elicited by the injection of DH-PS was over 10 folds of IL-1β, suggesting that DH-PS-induced anti-inflammatory activities might over-ride the inflammatory ones mediated by IL-1β. The signaling pathways of DH-PS-induced IL-1ra production was shown to involve ERK/ELK, p38 MAPK, PI3K and NFκB. Finally, we observed that IL-1ra level induced by DH-PS was significantly higher than that by F3, a polysaccharide extract isolated from another popular Chinese herbal medicine, Ganoderma lucidum. These results indicated that DH-PS might have potential applications for ameliorating IL-1-induced pathogenic conditions.
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影响因子：
3.5
作者：
Hsieh, Yves S. -Y.;Chien, Cheng;Wong, Chi-Huey
通讯作者：
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影响因子：
5.6
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Hua, Kuo-Feng;Hsu, Hsien-Yeh;Wong, Chi-Huey
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影响因子：
9.1
作者：
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通讯作者：
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影响因子：
5.3
作者：
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通讯作者：
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影响因子：
120.7
作者：
FISHER, CJ;DHAINAUT, JFA;STABLEIN, D
通讯作者：
STABLEIN, D