MoSec61β, the beta subunit of Sec61, is involved in fungal development and pathogenicity, plant immunity, and ER-phagy in Magnaporthe oryzae.
MoSec61β, the beta subunit of Sec61, is involved in fungal development and pathogenicity, plant immunity, and ER-phagy in Magnaporthe oryzae.
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DOI：
10.1080/21505594.2020.1848983
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发表时间：
2020-12
期刊：
影响因子：
5.2
通讯作者：
Liu XH
中科院分区：
文献类型：
--
作者：
Wei YY;Liang S;Zhang YR;Lu JP;Lin FC;Liu XH
蛋白质转运进入内质网的过程是所有分泌蛋白和许多可溶性细胞器蛋白生物合成的起始和决定性步骤。在这个过程中，Sec 61复合物是蛋白质转运的传导通道。在这项研究中，我们鉴定和表征的β亚基的Sec 61复合物在Magnaporthe Magnaporthe m与野生型菌株Guy 11相比，Δ Mosec 61 β突变体具有高度分枝的菌丝形态，分生孢子减少，对细胞壁完整性胁迫高度敏感，对水稻和大麦的毒力严重降低，限制了生物营养型入侵。Δ Mosec 61 β的膨压显著降低，影响了附着胞的功能。此外，Δ Mosec 61 β对氧化胁迫也很敏感，克服植物免疫的能力也有所降低。进一步的研究表明MoSec 61 β影响质外体效应子Bas 4和Slp 1的正常分泌。此外，Δ Mosec 61 β上调ER-吞噬水平。总之，我们的研究结果表明MoSec 61 β在M.米。
The process of protein translocation into the endoplasmic reticulum (ER) is the initial and decisive step in the biosynthesis of all secretory proteins and many soluble organelle proteins. In this process, the Sec61 complex is the protein-conducting channel for transport. In this study, we identified and characterized the β subunit of the Sec61 complex in Magnaporthe oryzae (MoSec61β). Compared with the wild-type strain Guy11, the ΔMosec61β mutant exhibited highly branched mycelial morphology, reduced conidiation, high sensitivity to cell wall integrity stress, severely reduced virulence to rice and barley, and restricted biotrophic invasion. The turgor pressure of ΔMosec61β was notably reduced, which affected the function of appressoria. Moreover, ΔMosec61β was also sensitive to oxidative stress and exhibited a reduced ability to overcome plant immunity. Further examination demonstrated that MoSec61β affected the normal secretion of the apoplastic effectors Bas4 and Slp1. In addition, ΔMosec61β upregulated the level of ER-phagy. In conclusion, our results demonstrate the importance of the roles played by MoSec61β in the fungal development and pathogenesis of M. oryzae.
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