The chicken transforming growth factor-beta 3 gene: genomic structure, transcriptional analysis, and chromosomal location.-猫眼课题宝

鸡转化生长因子-β 3 基因：基因组结构、转录分析和染色体定位。

DOI：

发表时间：

1995

期刊：

DNA and Cell Biology

影响因子：

3.1

通讯作者：

中科院分区：

生物学4区

文献类型：

作者：

D. Burt;B. R. Dey;I. Paton;D. Morrice;A. Law

在本文中，我们报告了鸡转化生长因子-β 3（TGF-β 3）基因的分离，鉴定和定位。该基因包含7个外显子和6个内含子，跨越鸡基因组的16-kb。人和鸡TGF-β 3基因5 '侧翼区域的比较揭示了两个序列保守区域。第一个包含ATF/CRE和TBP/TATA序列基序内的87 bp的区域。第二个是一个162 bp的区域，没有已知的序列基序。使用从各种胚胎和成人组织中分离的鸡RNA鉴定转录起始位点揭示了两个起始位点，P1和P2，其映射到这两个保守区域。鸡和哺乳动物TGF-β 3基因3 '侧翼区的比较也揭示了保守序列。最显著的同源性发现在转录区的3 '端。通过3 '-RACE分离的鸡TGF-β 3 cDNA的DNA序列分析显示，多个多聚腺苷酸化位点异常地远离poly（A）信号基序。Msc I限制性片段长度多态性（RFLP）标记用于将TGFB 3基因座定位到东兰辛参考回交的连锁群E7。与TH基因座的连锁表明TGFB 3基因座物理上位于鸡5号染色体上。

In this paper, we report the isolation, characterization, and mapping of the chicken transforming growth factor-beta 3 (TGF-beta 3) gene. The gene contains seven exons and six introns spanning 16-kb of the chicken genome. A comparison of the 5'-flanking regions of human and chicken TGF-beta 3 genes reveals two regions of sequence conservation. The first contains ATF/CRE and TBP/TATA sequence motifs within an 87-bp region. The second is a 162-bp region with no known sequence motifs. Identification of transcription start sites using chicken RNA isolated from various embryonic and adult tissues reveals two sites of initiation, P1 and P2, which map to these two conserved regions. Comparison of 3'-flanking regions of chicken and mammalian TGF-beta 3 genes also revealed conserved sequences. The most significant homologies were found in the 3'-most end of the transcribed region. DNA sequence analysis of chicken TGF-beta 3 cDNAs isolated by 3'-RACE revealed multiple polyadenylation sites unusually distant from a poly(A) signal motif. A Msc I restriction fragment length polymorphism (RFLP) marker was used to map the TGFB3 locus to linkage group E7 on the East Lansing reference backcross. Linkage to the TH locus showed that the TGFB3 locus was physically located on chicken chromosome 5.