Microbiological and 16S rRNA analysis of sulphite-reducing clostridia from river sediments in central Italy.
Microbiological and 16S rRNA analysis of sulphite-reducing clostridia from river sediments in central Italy.
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DOI：
10.1186/1471-2180-8-171
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发表时间：
2008-10-08
期刊：
影响因子：
4.2
通讯作者：
Mancini L
中科院分区：
文献类型：
--
作者：
Marcheggiani S;Iaconelli M;D'angelo A;Pierdominici E;La Rosa G;Muscillo M;Equestre M;Mancini L
微生物指标通常用于评估与淡水生态系统粪便污染有关的公共卫生风险。沉积物是微生物的储存库，因此可以提供过去污染事件的信息，而这是通过测试地表水无法获得的。此外，沉积物中存在的病原体可能是未来对人类健康的威胁。产气荚膜梭菌是一种典型的沉积物定殖菌，已被建议作为粪便污染的替代指标。为了适用于这种目的，微生物应广泛分布在污染的环境中。因此，本研究的目的是确定厌氧群落的组成，在台伯河下游盆地的沉积物样品，在意大利中部，通过一个组合的方法，涉及粒度分析的沉积物样品，以及微生物和分子（16 S rRNA）分析菌株。粒度分析表明，在大多数取样地点，粘土质沉积物的成分相似。微生物方法，采用，一个适应的标准方法，被证明是有效的分离厌氧菌从环境基质的遗传分析的目的。分离出83株细菌，并进行16S rRNA基因部分测序。而生化分析仅检测到C.系统发育分析表明，产气荚膜梭菌存在3个类群：C.产气荚膜梭菌C.双酶聚糖和B. cereus，包括8个类群。C.产气荚膜杆菌是几乎所有沉积物采样点中最常见的，在所有采样点和两个季节都存在（仅沿着台伯河和Aniene河进行了季节性采样）。没有一个描述的基因图谱显示与GenBank序列完全相似。本研究强调了C.产气荚膜杆菌作为河流沉积物中粪便污染的替代微生物指示剂。所有采样点和两个季节都存在这种细菌，再加上使用商业诊断试剂盒可以检测到这种细菌，这就证明了这一点。这项研究还表明，在台伯河下游流域存在着一个具有相当生物多样性的厌氧群落，其中C。产气荚膜梭菌作为其主要成分。的16S rRNA分析，同时确认孤立的物种之间的系统发育关系，也显示了单倍型模式不同于NCBI数据库中存在的。
Microbiological indicators are commonly used in the assessment of public health risks associated with fecal contamination of freshwater ecosystems. Sediments are a reservoir of microorganisms, and can thus provide information on past pollution events, not obtainable through the testing of surface water. Moreover, pathogens present in sediment may represent future threats to human health. Clostridium perfringens, a typical colonizer of sediments, has been suggested as an alternative indicator of fecal pollution. In order to be suitable for such purpose, the microorganism should be widely distributed in contaminated environments. The objective of this study was thus to determine the composition of the anaerobic community in sediment samples of the lower Tiber basin, in central Italy, through a combined approach involving granulometric analysis of sediment samples, as well as a microbiological and molecular (16S rRNA) analysis of strains. Granulometry showed a similar, clayey sediment composition, in most sampling sites. The microbiological method, employing, an adaptation of the standard method, proved to be effective in isolating anaerobic bacteria from the environmental matrix for the purpose of genetic analysis. Eighty-three strains of bacteria were isolated and the partial 16S rRNA gene sequenced. While biochemical analysis detected only C. perfringens strains, phylogenetic analysis indicated the presence of three clusters: C. perfringens, C. bifermentans and B. cereus, comprising eight taxa. C. perfringens, the commonest in almost all sediment sampling sites, was present in all sites, and in both seasons (seasonal sampling was carried out only along the Tiber and Aniene rivers). None of the described genetic profiles showed complete similarity with GenBank sequences. The study underlines the value of C. perfringens as an alternative microbial indicator of fecal contamination in river sediments. This is supported by the bacterium's presence in all sampling sites, and in both seasons, coupled with its detectability using commercial diagnostic kits. The study also illustrates the presence of an anaerobic community of considerable biodiversity in the lower Tiber basin, with C. perfringens as its main component. The 16S rRNA analysis, while confirming the phylogenetic relationships among isolated species, also showed haplotype patterns different from those present in the NCBI database.
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影响因子：
12.8
作者：
Ferguson, CM;Coote, BG;Stevenson, IM
通讯作者：
Stevenson, IM
DOI：
10.1099/00207713-44-4-812
发表时间：
1994-10-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
COLLINS, MD;LAWSON, PA;FARROW, JAE
通讯作者：
FARROW, JAE
影响因子：
4.4
作者：
Desmarais, TR;Solo-Gabriele, HM;Palmer, CJ
通讯作者：
Palmer, CJ
影响因子：
4.4
作者：
Hill, RT;Straube, WL;Colwell, RR
通讯作者：
Colwell, RR
影响因子：
2.3
作者：
Bezirtzoglou, E;Panagiou, A;Maipa, V
通讯作者：
Maipa, V