Timing within the menstrual cycle, sex, and the use of oral contraceptives determine adrenergic suppression of NK cell activity.
Timing within the menstrual cycle, sex, and the use of oral contraceptives determine adrenergic suppression of NK cell activity.
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DOI：
10.1054/bjoc.2000.1490
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发表时间：
2000-12
影响因子：
8.8
通讯作者：
Ben-Eliyahu S
中科院分区：
文献类型：
--
作者：
Shakhar K;Shakhar G;Rosenne E;Ben-Eliyahu S
据报道，涉及肾上腺素能机制的生理反应，如应激诱导的心血管指数变化，会沿着月经周期波动。据报道，手术后的转移发展也因原发性乳腺肿瘤切除时的月经期而异。自然杀伤（NK）细胞被认为在控制转移中起重要作用。我们最近在大鼠中的研究表明，在以高水平雌二醇为特征的发情期，肾上腺素能抑制NK活性和抗转移作用更为显著。在目前的人体研究中，我们检查了β-肾上腺素能激动剂，metaproterenol（MP）对NK活性的体外影响，比较了（a）在月经周期的3-4个不同阶段测试的女性（n = 10），（B）使用口服避孕药（OC）的女性（n = 10）和（c）男性（n = 7）的血液。在存在5种不同浓度MP（10- 8 M至10- 6 M）和不存在MP（基线）的情况下，评估每份血液样本中的NK活性。结果表明，MP对NK细胞抑制程度的显著组间差异：EC 50在黄体期比卵泡期低2.6倍，OC使用者比男性低1.8倍，后者对MP的影响最不敏感。在NK活性的基线水平上没有明显的组间差异或月经效应。这些发现为肾上腺素能对细胞免疫能力的影响提供了月经调节的第一个经验证据。这些研究结果的相关性乳腺癌切除的时间在月经周期和生存率之间的关系进行了讨论。© 2000年癌症研究运动http://www.bjcancer.com
Physiological responses that involve adrenergic mechanisms, such as stress-induced changes in cardiovascular indices, were reported to fluctuate along the menstrual cycle. Metastatic development following surgery was also reported to vary according to the menstrual phase during which a primary breast tumour was removed. Natural killer (NK) cells are believed to play an important role in controlling metastases. Our recent studies in rats demonstrated that adrenergic suppression of NK activity and of resistance to metastasis is more profound during oestrous phases characterized by high levels of oestradiol. In the current study in humans, we examined the in vitro impact of a β-adrenergic agonist, metaproterenol (MP), on NK activity, comparing blood drawn from (a) women tested at 3–4 different phases of their menstrual cycle (n = 10), (b) women using oral contraceptives (OC) (n = 10), and (c) men (n = 7). NK activity in each blood sample was assessed in the presence of 5 different concentrations of MP (10–8M to 10–6M), and in its absence (baseline). The results indicated marked group differences in the magnitude of NK suppression by MP: EC 50 was 2.6-fold lower in the luteal phase compared to the follicular phase, and 1.8-fold lower in OC users compared to men, who were least susceptible to the effects of MP. No significant group differences or menstrual effects in baseline levels of NK activity were evident. These findings provide the first empirical evidence for menstrual regulation of adrenergic impact on cellular immune competence. Relevance of these findings to the relation between the timing of breast cancer excision within the menstrual cycle and survival rates is discussed. © 2000 Cancer Research Campaign http://www.bjcancer.com
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影响因子：
2.7
作者：
LOZZIO, BB;LOZZIO, CB
通讯作者：
LOZZIO, CB
影响因子：
--
作者：
Beneke, R
通讯作者：
Beneke, R
影响因子：
3.3
作者：
Klokker, M;Secher, NH;Pedersen, BK
通讯作者：
Pedersen, BK
影响因子：
10.3
作者：
HRUSHESKY, WJM;GRUBER, SA;SIMMONS, RL
通讯作者：
SIMMONS, RL
影响因子：
2.2
作者：
OTTENHOF, PC;MORALES, A;BAINES, MG
通讯作者：
BAINES, MG