Immune challenge and satiety-related activation of both distinct and overlapping neuronal populations in the brainstem indicate parallel pathways for viscerosensory signaling.
Immune challenge and satiety-related activation of both distinct and overlapping neuronal populations in the brainstem indicate parallel pathways for viscerosensory signaling.
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DOI：
10.1016/j.brainres.2009.07.076
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发表时间：
2009-10-19
期刊：
影响因子：
2.9
通讯作者：
Goehler, Lisa E.
中科院分区：
文献类型：
--
作者：
Gaykema, Ronald P. A.;Daniels, Teresa E.;Shapiro, Nathan J.;Thacker, Gregory C.;Park, Su-Mi;Goehler, Lisa E.
关键词：
尾部脑干内脏感觉核将有关身体内部状态的信息传递到与进食行为和对免疫挑战的反应有关的前脑区域，并可能在免疫激活后调节摄取行为。疾病引起的食欲减退可能归因于“饱腹感”途径的增强、独立于饱腹感途径的独特“危险通道”的激活，或两者兼而有之。为了评估可能介导疾病对摄入行为影响的神经底物，我们分析了髓质神经元对首选食物（甜牛奶）消耗和腹膜内脂多糖挑战（减少甜牛奶摄入量）的响应模式和表型。脑干切片进行 c-Fos、多巴胺-β-羟化酶、苯乙醇胺-N-转移酶和胰高血糖素样肽-1 (GLP-1) 免疫反应性染色。加糖牛奶的摄入激活了孤束核 (NTS) 中的许多神经元，包括 NTS 尾半部的 A2 去甲肾上腺素能神经元。 LPS刺激激活了NTS中类似的神经元群，除了NTS中的头端C2肾上腺素能和中层A2去甲肾上腺素能神经元、腹外侧延髓中的许多C1和A1神经元以及背髓质网状核中的GLP-1神经元。 NTS 中 GLP-1 神经元激活数量的增加仅与甜牛奶摄入有关。平行处理的证据反映在臂旁核中，其中甜牛奶摄入导致内侧外侧部分、腹外侧部分和中央内侧部分的激活，而LPS攻击诱导外侧外侧部分中的c-Fos表达。因此，响应潜在危险的生理条件而产生的信号似乎是通过 NTS 和 VLM 中的特定儿茶酚胺能神经元群传播的，并且可能包括通过外侧 PBN 的通路。数据表明，免疫挑战涉及多种上行神经通路，包括独特的儿茶酚胺能“危险”通路，以及可能源自 NTS 的多模式通路。
Caudal brainstem viscerosensory nuclei convey information about the body's internal state to forebrain regions implicated in feeding behavior and responses to immune challenge, and may modulate ingestive behavior following immune activation. Illness-induced appetite loss might be attributed to accentuated “satiety” pathways, activation of a distinct “danger channel” separate from satiety pathways, or both. To evaluate neural substrates that could mediate the effects of illness on ingestive behavior, we analyzed the pattern and phenotypes of medullary neurons responsive to consumption of a preferred food, sweetened milk, and to intraperitoneal lipopolysaccharide challenge that reduced sweetened milk intake. Brainstem sections were stained for c-Fos, dopamine-beta-hydroxylase, phenylethanolamine-N-transferase, and glucagon-like peptide-1 (GLP-1) immunoreactivity. Sweetened milk intake activated many neurons throughout the nucleus of the solitary tract (NTS), including A2 noradrenergic neurons in the caudal half of the NTS. LPS challenge activated a similar population of neurons in the NTS, in addition to rostral C2 adrenergic and mid-level A2 noradrenergic neurons in the NTS, many C1 and A1 neurons in the ventrolateral medulla, and in GLP-1 neurons in the dorsal medullary reticular nucleus. Increased numbers of activated of GLP-1 neurons in the NTS was only associated with sweetened milk ingestion. Evidence for parallel processing was reflected in the parabrachial nucleus, where sweetened milk intake resulted in activation of the inner external lateral, ventrolateral and central medial portions, whereas LPS challenge induced c-Fos expression in the outer external lateral portions. Thus, signals generated in response to potentially dangerous physiological conditions seem to be propagated via specific populations of catecholaminergic neurons in the NTS and VLM, and likely include a pathway through the external lateral PBN. The data indicate that immune challenge engages multiple ascending neural pathways including both a distinct catecholaminergic “danger” pathway, and a possibly multimodal pathway derived from the NTS.
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影响因子：
15.1
作者：
Gaykema, Ronald P. A.;Goehler, Lisa E.
通讯作者：
Goehler, Lisa E.
影响因子：
2.9
作者：
Gaykema, Ronald P. A.;Chen, Chiao-Chi;Goehler, Lisa E.
通讯作者：
Goehler, Lisa E.
影响因子：
2.5
作者：
CECHETTO, DF;SAPER, CB
通讯作者：
SAPER, CB
影响因子：
3.2
作者：
Buller, KM;Day, TA
通讯作者：
Day, TA
影响因子：
2.4
作者：
Dunn, AJ;Swiergiel, AH
通讯作者：
Swiergiel, AH